两步控制策略优化生防菌B579芽孢生成条件（摘要）.pdfVIP

AgriculturalScience＆Technology，2011，12(2)：249—252 Copyright⑥2011，InformationInstituteofHAAS．Allrightsreserved PlantProtection Optimization ofSporulation Conditions ofBiocontrol ZHANGYan-chun，JIAJun-hui，ZHENGYu，CHENFang，ZENGMing-jie，LUOJian-mei，WANGMin KeyLaboratoryofIndustrialMicrobiology，MinistryofEducation，CollegeofBiologicalEngineering，TianjinUniversityofScienceandTechnolo— gY，Tianjin300457 Abstract fObjective]ForpreparingthebiopesticideofBacilluss曲 s『『B579withhighsporeconcentration，thesporulationconditionswereopti— mized．M『ethod1Two．stepfermentationcontroIstrategywasused，inwhich，thefirstphase(0—1Oh)wastoimprovecelIgrowth，andthesec- ondphase(1O 一3oh)wastopromotesporeformation．FourfactorsincludinginitiaIglucoseconcentration，fermentationpH．temperature (in lhesecondphase)andshakingspeed (inthesecondphase)wereoptimizedusingthemethodsofsinglefactortestandorthogonalexperiment． 『Result]TheinitiaIglucoseconcentrationshowedasignificanteffectonsporulation．TheoptimalconditionsforthesporeformationofB．subtilis B579wereasfollows：initiaIglucoseconcentration5g／L，fermentationpH7．0，thetemperatureforthefirstphase37oC andtheshakingspeed ， forthefirstphase1B0r／min，thetemperatureforthesecondphase40oC andtheshakingspeedforthesecondphase200 r／min；lnaddition． ， thefirstphasewas10 hand the second phase offermentationwasconductedfor30 h．Undersuchconditions．the sporeconcentrationand sporeformationratecouldreach9．43x1O CFU／mIand90．99％ ．respectively．whichrepresented6．70-foldand2．43-foldIncreasecompared withthosebeforeoptimization．iConclusion1ThesporesconcentrationofbiocontroJagentwasimprovedusingtwo-stepcontroIstrategy。which providedthebasisforbiopesticideproductioninlargescale． Keywords Bacillussubtilis：BiocontroIbacteria：Sporulat