植物OVATE蛋白分析.pdfVIP

’49_492 ACogpycruiguhTt④2d0。11c，eIIn＆foTrmecahtnioon’1nyst’it2u0te1ofH住AA4S‘．AIIrightsreserved．． Agrric—uIitura—『IBiJaasslic—Scclie1『ce一a一nl『dciMve—thc—ad ． AnalysisofOVATE ProteinsinPlants LIHong-ling ，HUANG Jian—ping CollegeofLifeSciences，NortheastAgriculturalUniversity，Harbin150030 Abstract OVATEproteinsareplantgrowthprofiling．OVATEhasbeen foundinrice，maize，Arabidopsis，Antirrhinum ，Tobaccoandsoon． ThispaperreportstheexpressionandfunctionofOVATEproteinindifferentplantssoastoprovidetheoreticalbasisforthefurtherresearchon Arabidopsis． Keywords Ovate；Arabidopsis；Tomato；Fruitshape Plantspeciesexhibitgreatdiversity jnorganshapeand boththepearshapeandtheelongatedfruitshape．Theovate size：alIkindsofplantshave differentcharacteris“cs infruit． genewasclonedrecentlyandshowntocorrespondtoanew 1eaf．stemsandroots．Alongwiththedevelopmentofmolecu- classofnucleus—localized．putativeregulatoryproteinsl ．The Iartechniques．itis found that the phenomenon ofthese geneisexpressedfrOm theearlystagesoffloraldevelopment plantsisdecidedbyaseriesofquantitativetraitIocusfQTL)． (10daysafterpollination)throughthefirsttwoweeksafter QTLscancontroIquantitativetraitgenesinthegenomeIoca— anthesis ．The mutation associated with a change fr0m tion．QTLssingleoriointlydetermine thecharacteristicsof roundto elongatedorpear—shaped fruitcreatesapremature plantsinmorphology．1nthestudyontomatofruit．aninterest- stopcodoninthesecondexon．This putative IOSS—of-function ing gene (ovate) was found．which was a plant-specific mutationisconsistentwiththerecessivebehavioroftheelon- gene．Kueta1．1lconstructednearly isogenicIinesforovate gatedorpear—shapedalleleingeneticstudiesl ． andshowedthatthe single Iocuscouldaccountforboth the