提高哺乳动物细胞医用蛋白质产量方法的研究进展.pdfVIP

第25卷第9期 生命科学 Vol. 25, No. 9 2013年9月 Chinese Bulletin of Life Sciences Sep., 2013 文章编号：1004-0374(2013)09-0937-05 ∙ 技术与应用∙ 提高哺乳动物细胞医用蛋白质产量方法的研究进展 * 王文强，闫　琦，侯　敢 ，黄迪南 (广东医学院生物化学与分子生物学教研室，湛江 524023) 摘　要：近年来，由于哺乳动物细胞具有对重组蛋白质进行正确折叠、装配和翻译后修饰的优点，利用哺 乳动物细胞生产医用蛋白质已成为生物制药产业的重要组成部分。但是利用哺乳动物细胞生产医用蛋白质 的产量仍然相对较低，因此，如何提高哺乳动物细胞医用蛋白质产量成为了研究热点。本文仅对构建高效 表达载体系统、筛选稳定高表达细胞株、优化转录及翻译过程、优化瞬时表达、改进培养工艺和抑制细胞 凋亡等几方面的研究进展作一综述。 关键词：哺乳动物细胞；表达载体；瞬时表达 ；培养工艺；细胞凋亡 Q952 TQ93 A 中图分类号： ； 文献标志码 ： Research progress on the improvement methods of medical protein production in mammalian cells WANG Wen-Qiang, YAN Qi, HOU Gan*, HUANG Di-Nan (Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China) Abstract: In recent years, because of the advantages of proper folding, assembling and the modification of post- translation for recombinant proteins, using mammalian cells to produce medical proteins has become an important part of biopharmaceutical industry. However, the yields of medical proteins in mammalian cells are still relatively low. Therefore, a hot research topic focuses on how to improve medical protein production in mammalian cells. In this review, we only summarized the recent achievements in methods for improving medical protein production in mammalian cells, including constructing high-level expression vector system, screening cell lines with stable and high expression, optimizing the transcription and translation, optimizing transient expression, improving culture biotechnolo