以AnnAGNPS 推估集水區泥砂產量可行性之研究.docVIP

这是 ftp://flysports.cc//att_doc%D3%D0%CB%E8%D6%E1%CD%BB%BA%E1%B6%CF%CB%F0%C9%CB%BA%F3%C4%C6%CD%A8%B5%C0%BE%DB%BC%AF%D7%B4%CC%AC%D1%D0%BE%BF.doc 的 HTML 档。 Google 在网路漫游时会自动将档案转换成 HTML 网页来储存。 这些搜索字词都已标明如下： 研究 有髓轴突横断损伤后钠通道聚集状态研究* 陈 菁△ 王燕 曾琳 龙在云 陈志强     （第三军医大学大坪医院野战外科研究所第六研究室，创伤、烧伤与复合伤国家重点实验室，               重庆 400042，中国）  摘要：目的 研究有髓轴突横断损伤后郎飞结区钠通道聚集状态的变化。方法 用雪旺细胞-背根神经元髓鞘化共培养系统复制周围神经髓鞘形成和郎飞结发育，于髓鞘化培养基中共培养第14 天用前房角切开刀造成有髓轴突横断损伤，在损伤后1、2、3、4、5、6、7、14天进行髓鞘碱性蛋白和钠通道免疫荧光染色，损伤前共培养作为对照。利用SPOT图像分析软件测量钠通道聚集簇的直径、长度和直径/长度比。结果 损伤前钠通道蛋白在有髓轴突郎飞结区形成直径/长度比略大于1的聚集簇；有髓轴突横断损伤后钠通道蛋白沿轴突纵向扩散，钠通道聚集簇的直径/长度比逐渐减小，损伤后第14天已无法检测到钠通道表达。损伤区出现节段性脱髓鞘。结论 轴突横断损伤可造成钠通道聚集簇扩散、消失，导致郎飞结结构破坏。 关键词：有髓轴突；横断损伤；钠通道聚集；郎飞结  中图分类号：R-332  R322.61 文献标识码：A Dynamics of Sodium Channels Clustering in Myelinated Axons after Transection* CHEN Jing△, WANG Yan, ZENG Lin, LONG Zai-yun, CHEN Zhi-qiang ( State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China ) ABSTRACT: Objective To investigate the dynamics of sodium channel clustering in the nodal region of myelinated axons after transection. Methods Myelinating cocultures of Schwann cell and dorsal root ganglion neuron were prepared to replicate myelination and the formation of node of Ranvier in peripheral nervous system. Axonal transection was carried out on myelinated axons in cocultures maintained under conditions promoting myelination for 14 days using a goniotomy knife under microscope guidance. Immunofluorescence staining of transected axons with anti-sodium channel antibodies and anti-myelin basic protein (MBP) antibodies were performerd 1, 2, 3, 4, 5, 6, 7 and 14 d after transection. Cocultures without transection were also immunostained with antibodies against sodium channel and against myelin basic protein to provide a control. The profiles parameters of sodium channel clusters such as diameter, length and diameter/length ratio were measured by means o