猪acta2 基因的克隆, 表达分析及其与生产性状的联系.pdfVIP

HEREDITAS (Beijing) 2009 5 , 31(5): 489―494 ISSN 0253-9772 研究报告 wygdDOI: 10.3724/SP.J.1005.2009.00489 猪ACTA2 基因的克隆、表达分析及其与生产性状的关联 1, 2 1 黄京书 , 熊远著 1. , 430070; 2. , 430064 为鉴定对猪生产性状有重要影响的新分子标记, 文章采用电子克隆结合 PCR 方法获得了猪肌动蛋白 α2(Actin alpha 2, ACTA2 )基因编码区序列和部分基因组序列,建立了第 2 内含子C1554T 替换的 PCR-Hin f Ⅰ- RFLP 基因分型方法,在所检测的 7 个不同猪群中除大白和梅大群体外,其他群体中均是 C 等位基因频率高于 T 等位基因的频率。标记与性状关联分析发现 ACTA2 基因型与肩部背膘厚、臀部背膘厚、肥肉率、瘦肉率、股 二头肌pH 和肌内脂肪显著或极显著相关,TT 基因型与CC 基因型相比具有更高的瘦肉率以及更低的肥肉率和背 膘厚。通过Real-time RT-PCR 分析发现ACTA2 在大白和梅山两个品种猪骨骼肌中的表达量都随着日龄的增加 而降低,在各个阶段,梅山猪中的表达量都比大白猪中的表达量高。 猪; ACTA2 ; PCR-RFLP; 表达谱; 关联分析 Cloning and expression analysis of porcine ACTA2 gene and its association with production traits 1, 2 1 1 HUANG Jing-Shu , XIONG Yuan-Zhu , DENG Chang-Yan 1. Key Laboratory of Swine Genetics and Breeding , Ministry of Agriculture , Huazhong Agricultural University, Wuhan 430070, China; 2. Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Academy of Agricultural Sciences, Wuhan 430064, China Abstract: To identify new DNA markers which have significant impact on pig production traits, the full coding sequence and partial genomic sequence of porcine ACTA2 (Actin alpha 2)gene were isolated using in silico cloning and PCR. PCR-Hinf -RFLP was developed to detect C1554T substitution in intron 2. The frequency of allele C is higher than that of allele T in all the seven detected pig populations except for Large White and Meishan×Large White. Association analysis of markers and production traits showed that the relation between ACTA2 gene and shou