进出口食品中天然甜味剂甜菊糖苷、甜菊双糖苷、甘草酸、甘草次酸及其盐类的测定英文意见稿.docVIP

Foreword This standard is prepared according to GB/T 1.1 -2009. This standard is proposed by and is under the charge of the Certification and Accreditation administration of the People’s Republic of China. This standard is drafted by Fujian Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China , Chinese Academy Inspection and Quarantine. The main drafters of this standard are Chen Jian, Yang Fang, Qian Jiang, Zhang Yuyan, Yu Kongjie. Please note that some of the content of this document may involve patent, The publisher of this document does not assume responsibility for identifying these patents Determination of stevioside, rebaudioside A, glycyrrhizic acid, glycyrrhetinic acid and their salts in foods for import and export 1 Scope The standard specifies the method of determination of stevioside, rebaudioside A, glycyrrhizic acid, glycyrrhetinic acid and their salts in foodstuffs for import or export. The method is applicable to determine stevioside, rebaudioside A, glycyrrhizic acid, glycyrrhetinic acid and their salts in Drinks, sauces, cakes, pickled vegetables and candy for import or export. 2 References This standard refer to the following standards and the latest versions are recommended: GB/T 6682 — Water for analytical laboratory use - Specification and test methods 3 Principle Extraction of the stevioside, rebaudioside A, glycyrrhizic acid, glycyrrhetinic acid and their salts with methanol?, and remove fat fromfat-bearing samples by nitrogen . After concentrated to a definite volume，it was analyzed and identified by HPLC-UV, quantified by external standards. 4 Reagents and Materials Unless otherwise specified, all regents used are A.R. grade, and water is the first grade according to GB/T 6682. 4.1 Phosphoric acid. 4.2 Acetonitrile: HPLC grade. 4.3 Methanol: HPLC grade. 4.4 Petroleum ether. 4.5 Anhydrous sodium sulfate：incandesce the anhydrous sodium sulfate 4 h at 650 ℃，and then stored in a sealed container. Phosphoric acid s