厌氧好氧交替快速筛选聚磷菌及其生理特性的研究.pdfVIP

中国环境科学 2012,32(10) ：1838~1844 China Environmental Science 厌氧/好氧交替快速筛选聚磷菌及其生理特性的研究 * 周明璟,纪树兰 ,崔丹红,秦振平 (北京工业大学环境与能源工程学院,北京 100124) 摘要：通过厌氧/好氧交替的平板筛选方法,快速的筛选出除磷率高于 60%的高效聚磷菌 15 株,并对其进 16S rDNA 和生理生化测定,除 2 株为芽孢杆菌外,其余均为 γ 变形菌纲,主要以 Klebsiella sp.和 Pseudomonas sp.为主.菌种除磷实验表明,聚磷菌除磷能力越高,发酵终点 pH 值越高.聚磷菌反投加实验表明,与活性污泥 52%的除磷率相比,投加后除磷率可达到 73.3%,可以有效提高活性污泥的除磷能力. 关键词：强化生物除磷；聚磷菌；菌种筛选；生理特性 中图分类号：X703 文献标识码：A 文章编号：1000-6923(2012)10-1838-07 * Quick screening of polyphosphate-accumulating strains and its characteristics. ZHOU Ming-jing, JI Shu-lan , CUI Dan-hong, QIN Zhen-ping(College of Environment and Energy Engineering, Beijing University of Technology, Beijing 100124, China). China Environmental Science, 2012,32(10)：1838~1844 Abstract ：A quick process was proposed to screen PAOs based on the aerobic/anaerobic cultivation. 15 strains, which had over 60% phosphate removal rate, were screened out and then tested by 16S rDNA and biochemical analyses. Except 2 strains of Bacillus sp., the other strains were γ-Proteobacteria, mainly Klebsiella sp. and Pseudomonas sp.. The phosphate removal experiment indicated that the pH values at the end of fermentation increased with the increase in the removal capacity of the PAOs. The phosphate removal rate increased from 52% to 73.3% after adding the PAOs strain screened into active sludge. The addition of the PAOs strain was an effective way to enhance the phosphate removal capacity of activated sludge. Key words ：enhanced biological phosphorus removal ；polyphosphate-accumulating organisms ；screening of strains； physiological characteristics 在生物法强化除磷(EBPR)工艺中,主要是应 率低、针对性较差、实验过程处理量大.本研究 用聚磷菌(PAOs)在厌氧释磷/好氧超量吸磷的生 通过厌氧/好氧