胃黏膜肠化生中H.pylori感染与π类谷胱甘肽转移酶表达的相关性.DOCVIP

临床医学论文-胃黏膜肠化生中H.pylori感染与π类谷胱甘肽转移酶表达的相关性 【摘要】? 探讨胃黏膜肠化生中幽门螺杆菌（H.pylori）致毒作用与以GSTπ为代表的人体对致癌物解毒系统间的相互作用。方法：利用SP法对219例胃黏膜活检组织进行GSTπ单克隆抗体的检测；利用HIDABpH2.5PAS粘蛋白组织化学技术对171例肠化生黏膜进行分型；利用HE及H.pyloriDNA PCR及ELISA方法对正常胃黏膜和肠化生黏膜进行H.pylori的检测。对80例H.pylori阳性患者进行H.pylori根除治疗。结果：正常胃黏膜未见GSTπ的表达，胃癌GSTπ阳性率为44.4%。H.pylori阴性组GSTπ阳性率高于H.pylori阳性组（P0.05）。H.pylori根除治疗后，根除组GSTπ表达高于未根除组（P0.05）。肠化生黏膜中GSTπ弱阳性或阴性表达如合并H.pylori感染者，胃癌发生的危险性增加。结论：在胃黏膜上皮肠化生阶段H.pylori的致毒作用与GST-π解毒作用彼此相互拮抗。 【关键词】? 肠上皮化生；胃癌；幽门螺杆菌 　　ＡｂｓｔｒａｃｔObjective：To study the relationship between the virulence of H.pylori and human carcinogen detoxification system in the stage of intestinal metaplasia (IM).Methods：About 219 cases were examined in the study, including 30 cases with normal gastric mucosa, 171 cases with IM and 18 cases with gastric cancer. The expression of GSTπ were detected by SP method. The types of IM were classified into Ⅰ,Ⅱ,Ⅲ by highiron diamine /alcian blue pH2.5/periodic acid Schiff (HIDABpH 2.5PAS)method：H.pylori infection was confirmed or excluded by hematoxylineosin (HE) staining, H.pyloriDNA PCR and enzymelinked immunosorbent assay (ELISA). Eighty cases with H.pylori infection were received the treatment of? H.pylori? eradication.Results：The GSTπ　expression was not found in normal mucosa, the positive rate of GSTπ in gastric cancer was 44.4%. The positive rate of GSTπ　expression in IM without H.pylori　infection was higher than that of IM with H.pyloriinfection (P0.05). The positive rate of GSTπ　expression in H.pylori eradicated group was significantly higher after H.pylori：eradication than before (P0.01). The risk of gastric cancer is increased if there was low or no expression of GSTπ　in H.pyloriinfected cases.Conclusion：The carcinogen detoxification role of GSTπ and the virulence of H.pylori might interact each other in the stage of IM, which is the precancerous condition of gastric cancer. 　　Key wordsGSTπ；intestinal metaplasia;gastric cancer;helicobacter pylori ??? 谷胱甘肽S转移酶（g