再生障碍性贫血患者几种造血相关免疫分子表达研究.pdfVIP

呈负相关;③骨髓中NO与以上各参数未见有相关性;④ 外周血及骨髓中CD31,CD44细胞数分别与外周血白细胞 数 (r=0.85,P0.O1;r=0.859,PO.01;r=0.865,PO.01; r=0.826,P0.01)、 血 小 板 (r=0.800,P0.01; r=0.808,P0.01;r=0.799,P0.01;r=0.754,P0.01)、 血 红 蛋 白 (r=0.815,P0.01;r=0.826,P0.01; r=0.846,P0.01;r=0.834,P0.01)以及骨髓中粒系比例 (r=0.460,P0.O1:r二0.649,P0.01;r=0.681,P0.O1: r=0.690,P0.O1)、红 系 LL例 (r=0.794 ,PO.01; r=0.764,P0.01;r=0.807,P0.01;r=0.828,P0.01) 和 巨核细胞数 (r=0.737,P0.01;r=0.735,PO.01; r=0.813,PO.01;r=0.752,PO.01)呈正相关。)~了 结论:再障患者G-CSF,TNF-a,N0,CD31和CD44的 改变可能与AA的发病有关。 关键词:贫血，再生障碍性 免疫分子 吞类 犯 Study on theExpression ofSeveral Haemotopoiesis-AssociatedImmunomoleculesin Patientswith Aplastic Anemia Abstract Objective:Expression ofseveralhaemotopoiesis- associated immunomoleculesinpatientswith aplastic anemia(AA)weredetectedtoanalyseitsrelationshipwith AA. Methods:Forthemarrowandperipheralbloodof20 AApatientsand8normalcontrols:① thelevelsofG-CSF andTNF-a weredeterminedwithELISAassay.② thelevel ofNOwithcolorimetrieassay.③theexpressionofCD31and CD44ontheMNCweremeasuredwithimmunofluorescence assay.(!)databasewasestablishedbyenteringdatatestedto computerandanalyzedbySASsoftware. Results:thelevelofG-CSF(P0.05)，TNF-a(P0.01) andNO (P0.01) inthemarrowandbloodwerehigherand theCD31+cellandCD44+cellwerelower(p0.01)inAA groupthannormalcontrolgroup.Bycorrelationanalysisin AAgroupitwasfoundthat:①theleveloftheG-CSFinthe marrowandbloodwasnegativelycorrelatedwiththatofthe leucocyteinblood (r=-0.558,P0.01;r=-0.419,P0.05) andthegranulocyteinmarrow (r=-0.553,P0.01:r=- 0.425,P0.05)respectively.② theleveloftheTNF-ainthe marrow andbloodandtheNO inbloodwasnegatively correlatedwiththatoftheleucocyte(r=-0.687,P0.01;r=- 0.797,P0.01;r=-0.516,P0.01)，platelet(r=-0.601, P0.01;r=-0.776,P0.01;r=-0.453,P0.05) and hemoglobin (r=-0.690,P0.01:r=-0.839,P0.01;r=-0.497, P0.01)inbloodandthegranulocyte(