5′-NT、ALP、ALT、γ-GGT联合检测对肝胆疾病、骨骼疾病的临床意义.DOCVIP

临床医学论文-5′-NT、ALP、ALT、γ-GGT联合检测对肝胆疾病、骨骼疾病的临床意义 ???????? 作者：李顺君，黄文方，饶绍琴 【摘要】? 目的? 检测各型肝胆疾病、骨骼疾病患者血清5′-核苷酸酶 (5′-NT)，谷丙转氨酶(ALT)和γ-谷氨酰转肽酶（γ-GGT），碱性磷酸酶 (ALP) 酶活性的变化，观察在肝胆疾病和骨骼疾病中的临床意义，同时与正常健康者作对照。方法? 用法国Biomerieux公司 (5′-NT)诊断试剂盒测定血清5′-NT酶活性，同时测定患者血清谷丙转氨酶(ALT)和γ-谷氨酰转肽酶（γ-GGT），碱性磷酸酶 (ALP) 酶活性的变化。结果? 发现在肝胆疾病和骨骼疾病时，血清5′-NT酶活性升高，尤其是急性黄疸型肝炎、肝癌、胆道疾病患者血清5′-NT酶活性差异有非常显著性（P＜0.01）时，随病情好转，血清5′-NT酶活性有所降低。结论? (1) 血清5′-核苷酸酶(5′-NT) 酶活性测定对诊断肝胆疾病、骨骼疾病具有重要价值。(2)联合检测血清5′-核苷酸酶 (5′-NT)，谷丙转氨酶(ALT)和γ-谷氨酰转肽酶（γ-GGT），碱性磷酸酶 (ALP) 酶活性的检测有助于鉴别诊断肝脏疾病、胆道疾病和骨骼疾病。??? ??? 【关键词】? 5′-核苷酸酶；谷丙转氨酶；γ-谷氨酰转肽酶；碱性磷酸酶 ；肝胆疾病；骨髓疾病??????? 【Abstract】? Objective? To study the serum? active enzymatic of? 5′-NT and ALT，γ-GGT? and ALP and in? hepatic? and? gall diseases，osteal diseases，compared? with the normal period as the control group. Methods? The serum? active enzymatic of? 5′-NT?? were examined using? Biomerieux? sa? Francais? Enzyline? 5′-NT? kit， together with? ALT，γ-GGT? and ALP .Results? It showed that the serum? active enzymatic of 5′-NT? in? hepatic and? gall? diseases，osteal diseases? levels were increase， especially? the more significant increase of the serum? active enzymatic of? 5′-NT? in level appeared in the patients with hepatic and? gall diseases，osteal diseases .It showed that the application of? 5′-NT? in acute hepatitis， live cancer and? osteal diseases? were significantly? higher than that of controls. The serum? active enzymatic of? 5′-NT may be useful in diagnosis and differentiation of some hepatocirrhosis and liver cancer.With the diseases getting better ，the application of? 5′-NT gradually decrease.Conclusion? (1) The serum? active enzymatic of? 5′-NT level was significantly correlated with types and? in hepatic and? gall diseases，osteal diseases.(2) serially observed? the serum? active enzymatic of 5′-NT? changing? in process of hepatic diseases? might be a biological prognostic marker， together with? ALT，γ-GGT? and ALP， it can be used to distinguish? and diagnose most hepatic and osteal di