LC%2fMS研究有机金属钌抗肿瘤药物与转铁蛋白的相互作用.pdfVIP

354 质谱学报 第3l卷增刊 LC／MS研究有机金属钌抗肿瘤药物与转铁蛋白的相互作用 郭 伟，罗 群，胡文兵，李鲜婵，熊少祥，汪福意 (中国科学院化学研究所，北京分子科学国家实验室，北京lool90) ofInteractionsbetween Ruthenium Investigation Organometallic Anticancer andTransferrinLC／MS Complexes by GUO Xian-chan,XIONG wei,LUOQull，HUWen-bing，LI Shao-xiang，WANGFu-yi National Molecular Sciences,Institute (Beijing Laboratoryfor ofChemistry, ChineseAcademyofScience,Bdjing100190,Ch／na) betweenkon-boundformshumanserum with Abstract：Re锄击∞sthe of transferring(Tr0organometallic rutheniumanticancer biphenyl(2)，∞= solutionI．￡／MScombinodwith and side-challl ethylenediamine)峨in戚酬inby trypsindigestionspecific under invitroat molar modifications．TheRu-Tff were conditions different complexesprepareaphysiological radiosfordifferentincubationstimes．Bothruthenium砌ewereshowntobindtosurfacehistidine complexes and residuesin the tobindto acid tyrosiae human％r哪transferrin’whilebiphe哪complexappears aspartic residuesin slafacetoo．Theseresults thatthealcne inthe ruthenium protein imply tigancl organometallic anticmlccr acrucialroleininteractionswith complexcsplays proteins． n吐henium sites Keywords：LC／MS；organometaUicanticancer唧l既器；tr．mfer衄interaction；binding 中图分类号：0657．63 文献标识码：A 文章编号：1004．2997(2010)增刊奶54北