固定化脂肪酶催化合成润滑油基础油聚酯的研究.pdfVIP

1固定化脂肪酶催化合成润滑油基础油聚酯的研究 刘文慧 陈必强 谭天伟* 邓利 王芳 (北京化工大学生命科学与技术学院，北京，100029) 摘 要：本文采用实验室自制的以织物为载体的固定化假丝酵母脂肪酶(Candida sp.99-125) ，来催化蓖麻油酸与多羟基醇 的聚合反应，成功合成出了网状聚酯。自制的固定化酶的价格远远比市售商品酶便宜，催化效率高，易于分离，具有 很高的实际应用价值。实验中采用红外光谱和凝胶色谱法（GPC ）对聚酯进行了表征；并且优化了聚合时间、温度、摩 尔比等对聚合反应的影响。在最适的反应条件下，成功获得了可以用于润滑油基础油的聚酯产品，其分子量为2000 Da～ 5000 Da 。 关键词：聚酯，假丝酵母肪酶，生物催化，润滑油 中图分类号：TQ 028.8 文献标识码：A Lipase-catalyzed Synthesis of Poly(ricinoleic acid) Star Polymers Wenhui Liu, Biqiang Chen, Tianwei Tan *, Li Deng, Fang Wang Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, P.R. China Abstract: Polyesters of poly(ricinoleic acid) and polyol acyl acceptors (trimethylolpropane and pentaerythritol), examples of lipophilic star polymers, were synthesized via bulk polymerization at 50 ℃ in a 3 to 8-day period, using lipases Candida sp.99-125. In the screening of several synthesis procedures, the highest molecular weight occurred when polyricinoleic acid were synthesized from trimethylolpropane mixed with ricinoleic acid using the monomer mole ratio of 1:5 by lipase Candida sp.99-125 as biocatalyst. The reaction result shows that silica gel have much effective as water-absorbent agent for polymerization of polyols and poly(hydroxy acids) compared to the reaction conditions open to the atmosphere. Such a procedure yielded trimethylolpropane - poly(ricinoleic acid) polymers with an average molecular weight from 2000 to 5000 Da, according to GPC analysis and the structure of the polymer were characterized by FT-IR, which could be satisfied for lubricant materials. Key words ：lipase; polyester; ricinoleic acid; polymerization; Candida sp.99-125 引 言 聚酯是相对新的一族合成润滑油基础油，它们定位与典