不同来源超氧化物歧化酶部分理化性质比较的研究.pdfVIP

安徽农业科学,Journal of Anhui Agri . Sci .2007 ,35( 15) :4426 - 4429 责任编辑 罗芸 责任校对 李洪 不同来源的超氧化物歧化酶部分理化性质比较研究 张彩莹 ( 南阳师范学院生命科学与技术学院, 河南南阳473061) 摘要 对4 种不 同来源的超氧化物歧化酶的部分理化性质进行 了比较研 究。结果表 明: 牛血 Cu ,Zn- SOD 和rhCu ,Zn- SOD 对一些化学试 剂的敏感性方面基本相似,rhCu ,Zn- SOD 的热稳定性最好,80 ℃水浴60 mi n 后 活力仍有56 % ; 在pH 5 .0 ～11.0 的范围内,Cu ,Zn- SOD 的活 力比较稳定, Mn- SOD 在pH 5 .0 以下容 易失活, 在碱性条件下较稳定。螺旋藻Fe- SOD 和rhMn- SOD 热、酸碱 的稳定性较低;Cu ,Zn- SOD 、Fe- SOD 和rhMn- SOD 对一些化学试剂和不 同变性剂的敏感性有明显的差别。rhMn- SOD 稳 定性, 对化 学试剂的敏感性方 面与天然原料来源 的 Mn- SOD 基本一致。Cu ,Zn- SOD 和 Mn- SOD 、Fe- SOD 相比较, 理化性质有 明显的差别。 关键词 Cu ,Zn- SOD;rhCu ,Zn- SOD;rhMn- SOD;Fe- SOD; 性质; 比较. 中图分类号 Q502 文献标识码 A 文章编号 0517 - 6611( 2007) 15 - 04426 - 04 Comparison Study on the Physico-chemical Characteristics of Superoxide Dismutase fromDifferent Sources ZHANG Cai- Ying ( College of Life Science and Technology ,Nanyang Nor mal University ,Nanyang , Henan 473061) Abstract In this paper , the physico- chemical characteristics of superoxide dismutase from different sources was studied . The results indicated :bovine Cu ,Zn- SOD was si milar to rhCu and Zn- SODin sensitivity to so me chemical reagents .The rhCu and Zn- SODther mostability was best , after 80 ℃ water baths 60 min its vigor still had 56 % .In pH 5 .0 ～11 .0 scopes , Cu and Zn- SOD vigor was quite stable ;rhMn- SOD below pH 5 .0 easy deactivation ,was stable under the alkalinity condition .The stability of Spiral algae Fe- SOD and rhMn- SOD at heat , acid and alkali was lower .There was obvious difference in sensitivity to some che mical reagents and the different denaturant sensitivity for Cu and Zn- SOD,Fe- SOD and rhMn- SOD.The rhMn- SOD stability , to the chemical reagent sensitive was consistent to Mn- SOD which origi nated with the natural raw material .The physico- che mical characteristics of Cu ,Zn- SOD and Mn- SOD,Fe- SOD ha