成纤维细胞生长因子10 mRNA在寻常型银屑病皮损中的表达_医学论文.docVIP

成纤维细胞生长因子10 mRNA在寻常型银屑病皮损中的表达_医学论文 成纤维细胞生长因子10 mRNA在寻常型银屑病皮损中的表达_医学论文 【关键词】 银屑病,成纤维细胞生长因子10,,原位杂交 　　Expression of fibroblast growth factor 10 mRNA in epidermal lesions of psoriasis vulgaris 　　【Abstract】 AIM: To study the mRNA expression of fibroblast growth factor 10(FGF10) in psoriasis vulgaris and the role of FGF10 in the pathogenesis of active psoriasis. METHODS: We detected FGF10 mRNA in normal epidermal tissues from 22 normal volunteers undergoing cosmetic surgery, uninvolved and psoriatic lesions from 22 patients by in situ hybridization. RESULTS: The expression of FGF10 mRNA in epidermis specimens was found in psoriatic lesion，uninvolved and normal epidermis with in situ hybridization. Positive staining in epidermis was distributed in the whole epidermis, and was observed in cell plasma(95%), while positive staining in normal epidermis was localized mainly at the basal cell layer(5%). Positive staining in uninvolved epidermis was located in the basal layer of epidermis (82%).Compared with the normal epidermis, the positive rate and the mean expression level of FGF10 mRNA in epidermis of psoriasis vulgaris were significantly higher than those in the normal epidermal tissues(0.05). CONCLUSION: The higher lever of FGF10 mRNA in the epidermis of patients with psoriasis vulgaris may play an important role in the early pathogenesis of psoriasis vulgaris. 　　【Keywords】 Psoriasis Fibroblast Growth Factor 10 In situ hybridization 　　【摘要】目的： 研究成纤维细胞生长因子10 mRNA在寻常型银屑病皮损中的表达，探讨其在银屑病发病中的作用机制. 方法： 应用原位杂交法检测FGF10 mRNA在22例正常皮肤组织、银屑病皮损和未受累皮肤中的表达和分布. 结果： 原位杂交法检测发现，在22例正常皮肤组织、银屑病皮损和未受累皮肤表皮的全层或表皮中下层可见FGF10 mRNA表达(95%)在正常皮肤表皮的基底层或少量细胞内可见FGF10 mRNA的表达(5%)在寻常型银屑病非皮损表皮的中下层或个别细胞内可见FGF10 mRNA的表达(82%). 寻常型银屑病皮损表皮中FGF10 mRNA的表达明显高于正常对照组(0.05). 结论： FGF10 mRNA在银屑病发病早期阶段的表达增高可能与银屑病的早期发病有关.　　【关键词】 银屑病 成纤维细胞生长因子10 原位杂交 　　0引言 　　银屑病组织病理学的特征性表现为角质形成细胞异常增殖、真皮浅层淋巴细胞浸润、真皮乳头毛细血管迂曲扩张. 研究表明，在一些生长因子的作用下，角质形成细胞可表现为异常增殖和分化异常，如表皮生长因子、转化生长因子α和成纤维细胞生长因子等，其中成纤维细胞生长因子10（Fibroblast growth factor 10, FGF10）对角质形