大肠埃希菌gyrA、parC和marOR基因突变与喹诺酮耐药的相关性.pdfVIP

346 喹诺酮耐药的相关性 王世瑜张坚磊蒋萍陈锦艳穆红彭林 摘要 目的：研究大肠埃希菌gyrA、parC和rnarOR基因突变与喹诺酮类耐药的相关性。方法：采用微量稀释法 进行常规药敏试验，筛选3株萘啶酸敏感大肠埃希菌和37株萘啶酸耐药大肠埃希菌株；PCR扩增大肠埃希菌喹诺 验结果进行比较，研究其相关性。结果：37株耐药株均出现gyrA基因突变，但对环丙沙星低耐株最低抑菌浓度 个位点突变，parC基因出现单位点突变；在环丙沙星高耐株(MIC=256ms／L)，并伴有其他类抗菌药物的多重耐药时， 埃希菌对喹诺酮耐药中起着重要作用，gyrA和parC基因突变的程度与大肠埃希菌耐药水平有关，marOR基因多位 点突变在多重耐药机制中具有一定的作用。 关键词大肠杆菌喹诺酮类基因 突变多耐药相关蛋白质类 betweenQuinoloneResistanceand and／ormarORGene Relationship gyrA，parC MutationsinEscherichiaCoiI Jianlei，JIANGPing，CHENJinyan，MUHong，PENGLin WANGShiyu，ZHANG Department between and and，orrn口OR Abstract the resistance Objective：Tostudyrelationshipquinolone gym，parC gene withmicrodilution were mutationinEscherichia ofE．coilwereselected method，which coil(E．coil)．Methods：Fo啊strains seven the threenalidixic nalidixicacid—resistant(NALR)．In acid—susceptible(NAIJs)andc}lirty meantime，quinolone were PCR．ThePCR ofmozORwe陀 resistance-determiningregions(QRDR)ofgyrAandparCamplifiedby products region other strainswereselectedwithdirect todetectthe resistance sequenced possiblegenechanges．The sequencing．The and marORmutationinE．coilwasstudied．Results：The between resistance quinolone gyrA，par(?and／orgene relationship theNALBst