妊高症模型母鼠胎盘超声造影定量血流灌注参数和病理微血管密度对比分析.pdfVIP

))，其与 i/侧脑室 妊高症模型母鼠胎盘超声造影定量血流灌注参数与病理微血管 密度对比分析 许杨青1陈欣林1路小军1杨小红1陈佩文1 黄君红1胡蕾1黄程1 陆兆龄2 1.湖北省妇幼保健医院超声科 2.北京和睦家医院 摘要 目的：探讨妊高症模型母鼠胎盘超声造影(CPS)定量血流灌注参数与肿瘤病理微血管 密度(MVD)的相关性。 方法：给34只妊高症母鼠行超声造影(CPS)， 造影后应用Sonoliver1.025分析软 件定量分析软件绘制胎盘的造影时间一强度曲线(TIC)，得出妊高症模型母鼠造影定 量血流灌注参数达峰时间(TTP)、峰值强度(IMAX)、曲线下面积(AUC)及总灌注量(V)； 以上所得参数与造影后病理组织免疫组化染色计测的MVD对照，进行相关性分析。 关键词：超声检查；妊高症大鼠；定量分析；微血管密度 ComparativeStudyonQuantitativeParametersofBloodPerfusionand MicrovesselDensityinPlacentaofRatmodelofPreeclampsiaEvalutedwith Contrast-enhancedUltrasound XUYang-qing1CHENXin-lin1* LUXiao-jun1YANGXiao-hong1CHENPei-wen1 HUANGJun-hong1HULei1HUANG-Cheng1 LUZhao-ling2 1.DepartmentofUltrasound,HubeiMaternalChildHealthHospital,Wuhan, 430070,China;2.UnitedFamilyHospitalsClinics,Beijing,100000,China Abstract Objective:Toinvestigatethecorrelationbetweenquantitativeparameters withcontrast-enhancedultrasound(CEUS)andmicrovesseldensity(MVD) Methods: TwentyplacentaofratmodelofPreeclampsiaunderwentCEUS examination. Thepeakintensitytimecurvesontheenhancedimageswere analyzedquantitativelywithcomputertogetQuantitativeParameters(Themean transittime,themaximalpeakintensity,theareaundercurveandthemean perfusionvolume) TheseparameterswerecomparedwithMVDcountedwith immunohistochemistryandthecorrelationwasstatisticallystudied. Results:Thepeakintensitytimeintheenhancedimageswas(28.55±11.45) s,themeantransittimewas(134.77±56.45)s,themaximalpeakintensity was(84.83±20.15)dB,theareaundercurvewas(7102.61±245.76)dB,Themean perfusionvolumewas(58.01±23.56)dB,andtheMVDofplacentaofratmodelof Preeclampsiawas(230.98±34.78)in20rats. ThemaximalpeakintensitywascorrelatedpositivelytoMVDofhistological section(r=0.885,P＜ 0.01).Theareaundercurvewascorrelatedpositively toMVDofhistologicalsection(r=0.883,P＜ 0.01).Themeanperfusionvolume wascorrelatedpositivelytoMVDofhistologicalsection(r=0.877,P＜ 0.01). TherewasnocorrelationbetweenthepeakintensitytimeandM