拮抗真菌菌株LV-12与Zh-20的鉴定及生防作用的初步研究.pdfVIP

拮抗真菌菌株LV．12和Zh．20的鉴定及生防作用的初步研 究 秦刚1,2,3曾大兴2吴庆丽1·3姜子德1 (1．华南农业大学生物防治教育部工程研究中心，广州510642；2．深圳职业技术学院化生学院，深圳 1130) 518055；3．成都农业科技职业学院，成都61 摘要：从广东省采集的绿巨人褐腐病病株分离筛选到2株具有生防活性的真菌菌株LV-12和Zh-20，依据形态特征并结 Schoultieseta1．)n平板对峙培养显示： 合ITS序列同源性分析将其鉴定为苞叶芋柱霉(CylindrocladiumspathiphyUi LV-12和zh．20对香蕉炭疽菌、立枯丝核菌和群结腐霉等重要病原真菌具有明显的拮抗作用，抑菌率分别为46．5％～ 53．9％和42．2％～48．7％。 关键词：生物防治：苞叶芋柱帚霉；ITS序列分析 IdentificationandCharacterizationofBiocontrolStrainLV-12andZh一20 Fungal Zidel QINGan912r，ZENGDaxin92，wUQinglil一，JIANG ResearchCenter China rJ．Engineering ofBiologicalControl,MinistryofEducation，SouthAgricultural 510642，China；2．School and University,Guangzhou ofAppliedChemistryBiologicalTechnology, Shenzhen Vocational Scienceand Polytechnic,Shenzhen518055,China；3．ChengduCollegeofAgricultural 611130,China) Technology,Chengdu of and thediseased in Abstract：Twoisolates fungiCLV-12Zh一20)from plant(Spathiphyllumpallas)collectedGuangdong has identifiedas Schoultiesela1．basedontheir Province，Whichantifungalactivity，were Cylindrocladiumspathiphylli characteristicsandITS resultsofdualculturetestsindicatedthatLv-12andZh-20were morphological sequences．The very to and effectswere46．5％～ Colletotrichummusae，RhizoctoniasolaniPythium etc．。whichinhibitory antagonistic myriotylum 53。9％and 42，2％～48．7％respectivel