IgY联合乳酸杆菌防治阴道白色念珠菌感染的实验研究.pdfVIP

142 2006 2 35 2 IgY 江西赣南医学院病原生物学教研室( 赣州341000) 　张文平　马廉兰　谢水祥　傅颖媛* 目的: 观察特异性抗白色念珠菌IgY 和乳酸杆菌的体内外生物学效应, 探讨将 二者复 应用的可能性。方法: 分离人阴道上皮细胞, 用抗白色念珠菌IgY 和乳酸杆菌对阴 道上皮细胞预处理后进行白色念珠菌黏附抑制试验; 建立小鼠白色念珠菌性阴道炎模型, 通 过阴道局部给抗白色念珠菌IgY 和乳酸杆菌来观察其疗效。结果: 抗白色念珠菌IgY 和乳酸 杆菌均能抑制白色念珠菌黏附阴道上皮细胞, 并能减少白色念珠菌在小鼠阴道内的定植和 减轻小鼠阴道局部的炎症反应。结论: 特异性抗白色念珠菌IgY 具有良好的体内外生物学效 应且优于单独使用乳酸杆菌, 二者复 应用效果最佳。 念珠菌, 白色/ 药物疗法　乳酸杆菌/ 治疗应用　@ 抗白色念珠菌IgY Astudyof the effects of IgYand Lactobacillusonthe prevention andtreatmentof vaginal infection dueto Candidaalbicans Department of Pathogenic Biolog of Gannan M edical College ( Gangzhou 34100) 　Zhang Wenping　M a Lianlan　Xie Shuixiang　et al : - - 　　ABSTRACT　Objective T o observe the bio act ivit of ant i Candida albicans IgY and Lact obacillus . : in vivo and intro and to stud the possibilit of combined application of them Met hods The human vaginal epithelium cells w ere isolated and adherence inhibition experim ent w as performed after pret reat ing C. albicans and buccal Epithelium cells w ith ant i-C. albicans IgY and Lactobacillus. After t he mouse model of . , - . vaginitis infected w it h C albicans therapeutic effects of anti C albicans IgY and Lact obacillus . : - . administered vaginall w ere observed Results Anti C albicans IgY and Lact obacillus can inhibit adherence of Candida albicans to vaginal epithelium cells and reduce the vaginal C. albicans colonizat ion as w ell as alleviate t pical signs of mouse w ith C. albicans colonization. Conclution: Anti-C. albicans IgY is of good biological effect against Candida albicans in vivo and intro ,