新缰加工番茄上番茄花叶病毒分子鉴定.pdfVIP

新疆农业科学 2008, 45( 3) :484- 489 Xinjiang Agricultural Sciences 新疆加工番茄上番茄花叶病毒的分子鉴定 1 1 1 1 2 姜玉霞 , 向本春, 安仙丽, 黄家风 , 汪铖华 ( 1. 石河子大学农学院, 新疆石河子 832000;2. 新疆康正农业科技开发 限责任公司, 乌鲁木齐 830000) : xj124, 1 ToMV CP RT- PCR , 689 bp PCR pMD18- T TOP10 , PCR 689 bp cDNA , 689 bp ToMV CP , 1 480 bp (OR ) , 160 , ToMV CP xj124 ToMV CP , 84.4% ~ 99. 8% , 98. 7% ~ 100% : ; ; ; :S4361412119 :A : 1001- 4330(2008) 03- 0484- 06 Identification on Molecular of Tomato mosaic virus of Processing Tomato in Xinjiang 1 1 1 1 2 JIANG Yu- xia , XIANG Ben- chun ,AN Xian- li ,HUANG Jia- feng ,WANG Chen- hua (1. College fo Agronomy, Shihhotze University, Shihhotze Xinj iang 832000, China; 2. Xinj iang Kangzheng Agricultural Science and Technology evelopment Co.Ltd., Urumqi 830000, China) Abstract: Xj124 isolate was obtained from naturally infected processing tomatoes with symptoms of mosaic and malformation in Xinjiang. A pair of primers was designed based onToMV CP gene, and a fragment of about 689 bp was amplified by RT- PCR. The products of PCRwere cloned into PMD18- T and transformed into TOP10. The combined clone was identified by restriction enzyme digesting and the nucleotide sequencing. The results showed that the cloned segment was 689 bp and contained one open reading frames ( OR ) that was composed of 480 bp nucleotides encoding a coat protein gene of 160 amino acids. Comparison of the nucleotide and amino acid se uences of the full length of CP gene of ToMV xj124 with the corresponding se uences of the part of the ToMV isolates reported showed that the homology is 83. 1%- 99. 1% at nucleotide level ,