CB法微核实验检测细胞DNA损伤修复能力.pdfVIP

COSHA 第二十届海峡两岸及香港、澳门地区职业安全健康学术研讨会论文集 CB法微核实验检测细胞DNA损伤修复能力 程学美 山东省疾病预防控制中心 【摘要】 目的 探讨一定剂量的x射线照射后双核淋巴细胞微核率作为检测细胞DNA损伤修复能力 生物学指标的可行性。方法 采用CB法微核实验，检测人外周血离体培养24小时后以0.5 ~ 4 Gy 剂量x射线照射后的微核率，计算修复能力指数（DRC），建立以公认的辐射损伤模型和双核微核 为观察终点的检测细胞DNA损伤修复能力的一种新方法。 结果 CB法微核实验结果显示照射剂量 和淋巴细胞微核率存在较紧密的相关性。随着辐射剂量增加，细胞微核率增大，细胞内微核个数增 多，细胞死亡数亦增加。0.5 Gy剂量x射线照射后微核率明显增大，与0 Gy剂量组（对照组）相 比，差异有显著性（t=2.64，p=0.03），阅片结果显示，两组实验的细胞死亡数较少，差异不明 显。结论 从微核率，微核个数，死亡细胞数综合考虑，在本实验条件下，可选择0.5 Gy剂量x射 线照射以检测细胞DNA损伤修复能力大小。 【关键词】 CB法微核实验；X射线；DNA修复能力 Detecting Cell Damage Repair Ability using Cytokinesis-block Micronucleus Method Cheng Xuemei, Qin Xiaomei, Liu Kun, Zhang Tianliang, Shan Baode. Shandong Center for Disease Control and Prevention, Jinan 250000, China Corresponding author: Shan Baode, Email: cdcbaodesh@163.com 【 】 Abstract : Objective To discuss feasibility for reguarding micronucleus rate of binucleus lymphocytes after x rays radiation as biomarker detecting cell DNA damage repair ability. Method Using cytokinesis- block micronucleus method, to acquire micronucleus rate of binucleus lymphocytes after x rays radiation which from 0.5 ~ 4 Gy dose, then to calculate DNA Repair Capacity (DRC) index, we could establish one new method detecting cell DNA repair ability on the base of radiation damage model and binucleus micronucleus lymphocyte as terminal biomarkers. Result The dose of x ray radiation is obvious related to binucleus lymphocytes mironucleus rate. The micronucleus rate and the number of micronucleus increase as radiation dose raising, and the number of dead cells also goes up. The micronucleus rate rises with 0.5Gy dose x ray, and difference of the rate between 0.5 Gy radiation group and 0 Gy control group is evident (t=2.64, p=0.03), then the number of dead cells between the groups is both small, without difference. Conclusion In condition of the laboratory