高效亲和色谱法测定2种中药成分和人血清白蛋白的结合率.pdfVIP

2011 4 Vo.l 29 No. 4 April 2011 Ch inese J ourna l of Chromatograp hy 358~ 361 研究论文 DOI: 10. 3724 /SP. J. 1123. 20 11. 00358 2 1 2 1 1 1* 1 蔡晓明, 张 岩, 于 龙 , 郭志谋, 张秀莉 , 梁鑫淼 (1. , , 116023; 2. University of CalifoniaIrvine, CA 926974625, USA) : (HPAC) (HSA) HSA , H SA , HSA HSA 1026% 1020% H SA 1425% , HPAC, : ; ; ; ; ; : O658 : A : 100087 13(2011 Detection of drughuman serum album in binding ratios of two Chinesem edicinal ingredients by high performance affinity chromatography 1 2 1 1 1* 1 CAI Xiaom ing , ZHANG Yan, YU Long , GUO Zhmi ou, ZHANG X iuli , LIANG X inm iao (1. CAS K ey L aboratory of Separation S ien e for An aly ti al Chem istry , D alian In stitu te of Chem i a l Phy si s, Chin ese A adem y of S ien es, Dalian 116023, Ch in a; 2. Un iver sity of Ca lifon iaIrvin e, CA5, U SA ) Abstract: The interaction of two Chinese me icinal ingre ients an human serum album in (HSA ) has been investigate by high performance affinity chromatography (HPAC). HSA boun e silicabase stationary phase w as prepare base on the click chem istry strategy, an packe in a column (name as HSA column). The rugHSA bin ing ratio was calculate from the ifference of the rug s retention tmi es on theHSA column an silica column (blank column). The warfarinHSA bin ing ratio eterm ine by this metho was smi ilar to the refer ence reporte value by ultrafi