山楂黄酮的薄层色谱分离鉴定的研究_梁淑芳.pdfVIP

2 4 Vol. 2 No. 4 200 12 Chemistry and Industry of Forest Products Dec. 200 梁淑芳, 马耀光, 马柏林 ( 西北农林科技大学生命科学学院, 陕西 杨凌712100) LIANG S F : ( Cr ataeg us p innatif ida Bge.var . maj or N. E. Br) , 7 , 7 , 415 , : ; ; ; : Q949.751. 8; O658. 64 :A : 025 - 2417( 200 ) 04- 0086- 0 A STU DY ON SEPARAT ION AND IDENT IFICAT ION OF FLAVONOIDS IN CRA TA E G US PINN A TI FIDA BGE. BY THINLAYER CHROMATOGRAPHY LIANG Shufang, MA Yaoguang, MA Bailin ( L if e Science Co age, N orthw est ScienceTechno ogy Univ ers ity of A gr icu t ur e and For est ry , Yang ing 712100, China) Abstract: T his paper studied new method of separation and identification of flavonoides in Crataeg us p innatif ida Bge.var. maj or N. E. Br . Influence of microemulsion types on pow er of resolving flavonoids was invest igated w ith SDS/ nC H OH/ nC H / H O microemulsion as eluant on polyamide film. The flavonoids were separated entirely. 4 9 7 16 2 Seven flavonoids spots w ere obtained respectively from leaves and fruit, but they are not identical. Compared w ith the general mobile phase composed of C H OHCH COOHH O= 415 the sensitivity of detection w as improved markedly 4 9 2 and the results w ere satisfactory. This new chromatographic method is simple, accurate for separation and ident ifica tion of flavonoids in C. p innatif ida Bge.var. maj or N. E. Br . Key words: Crataegus p innatif ida Bge. var. maj or N. E. Br. ; flavonoids; thinlayer chromatography; microemul sion , , 94. 4 % , 85 %, 62. 5 % ,