梅山猪、大白猪与梅大杂交猪背最长肌中差异表达的14个表达序列标签的分离、鉴定及组织表达分析(英.pdfVIP

业生物技术学报 Journal of Agricultural Biotechnology 2006,14 (3): 323~328 ·研究论文· Isolation, Identification and Expression Profile of Fourteen Differentially Expressed Sequence Tags in the Longissimus Dorsi Muscle Tissues from Meishan, Large White and Meishan Large White Cross Pigs * LIU Yong-gang, XIONG Yuan-zhu**, ZUO Bo, JIANG Si-wen, DENG Chang-yan, LI Jia-lian, LI Feng-e, ZHENG Rong In order to detect the molecular mechanism of the heterosis in pigs, mRNA differential display (DD) technique was performed to investigate the differences in gene expression in the muscle tissues from Meishan, Meishan Large White cross and Large White pigs. Fourteen expressed sequence tags (ESTs), differentially expressed between the hybrids and purebreds, were isolated and identified through semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The nucleotide sequence analysis revealed that the 14 ESTs were not homologous to any of the known genes or ESTs. These novel ESTs were then deposited into GenBank database. Tissue expression profile analysis showed that the ESTs were expressed in most of tissues including heart, spleen, liver, kidney, small intestine, ovary and lung, and this also implied that these genes might be important for the life process. Our results indicate the diversity of differential display directions of genes between the hybrids and purebreds in the Meishan Large White cross combination. Results also suggest that the heterosis in pigs might be derived from the differential expression of many