《RP-HPLC法同时检测苹果汁中5-HMF、展青霉素、乐果及多菌灵》.pdfVIP

I竺兰兰兰竖 分析栓涮‘。 ’ Food Sciz嬲and：technologyof Industry 、 ‘‘ “ ‘ ’ ’。…。+ …1’’’’‘ ’，‘， otR P—H PLG法同时检测苹果汁 5一HMF、展青霉素、乐果及多菌灵 牛鹏飞，纨农学‘，苏海洁 (陕西师范欠学食品工程系，陕西西安710062) 接要：免霹睁定蘩捡测苹装汁中5一羟甲基猿醛(5-HMF)、展黄霉素、乐暴麓多蘧灵黪舍薰，建立了一静基于多遴蘧 紫外徐溅器的葭糖离效液相色谱(RP-HPLC)分离方法。以甲醇：乙耩：拳=l：】：8的混合液作为流动穗，采用e。色谱 柱，在流速0．8mL·min～、温腱30℃下进行5一HMF、展青霉素、乐暴及多茵萸的色谱分析。结果表明：前7min内， 应用于同时定量检测苹果浓缩汁中5一HMF、展青霉素、乐果及多茵蔻。 关键词：Rp-HPLc，5一HMF，展膏霉素，乐果，多菌灵，苹恭汁 Simultaneousdeterminationof and 5一HMF，patulin，dimethoate carbendazolinapplejuicebyRP-HPLC NIU Nong-xue+，SUXiao--jie Peng-fei，Q112 ofFood Normal (DepartmentEngineering，ShaanxiUniversity，)ci’觚710062，China) Abstract：Areversed—phasehigh—performanceliquid methodwith and ultravioletdetectorwas todeterminethe carbendazolin developed 5一HMF，patulin。dimethoateapplejuice mobile wasamixed at simultaneously．Thephaseemployed aflow—rateof columnat30℃fofthewholerun．5-HMFand Cl#chromatographic patulin 0．80mL·min～through 278nm,Carbendazo{andelutedin 30min wereelutedin7minandweredetectedat dimethoatewere the following andweredetected210nm．The of a11within0．5％ at precisionpeakarea(n=10)were