急性缺氧对大鼠心肌组织AMPK磷酸化和糖原含量的影响.docVIP

急性缺氧对大鼠心肌组织AMPK磷酸化和糖原含量的影响 单发波，蔡明春，徐 刚，杨 涛，殷 骏，黄庆愿 （400038重庆，第三军医大学高原军事医学系病理生理学与高原生理学教研室，教育部高原医学重点实验室，全军高原生理和高原病研究重点实验室） [摘要] 目的 研究急性缺氧对大鼠心肌组织AMPK磷酸化和糖原含量的影响，探讨急性缺氧时心肌组织的能量代谢状况。方法 用随机数字表将16只健康雄性SD大鼠分为平原对照组（n=8）和急性缺氧组（n=8）。急性缺氧组大鼠置模拟海拔5000m高原的低压舱中。24h后测定2组大鼠血糖、血乳酸以及心肌组织糖原含量，用心导管技术测定大鼠心脏功能，Western blot法检测心肌组织AMPK、磷酸化AMPK（pAMPK）水平以及葡萄糖转运体-4（GLUT4）的表达。结果 与平原对照组相比较，急性缺氧组大鼠肺动脉压显著升高，右心室功能显著增强，左心室功能无明显变化；血糖含量显著升高[（5.6±0.6）mmol/L vs （7.5±0.5 ）mmol/L, P0.05]；左、右心室心肌组织GLUT4表达显著增强，糖原含量显著增加[右心室心肌：(4.78±1.29) mg/g vs (6.55±1.20) mg/g，P0.05；左心室心肌：(4.15±0.77) mg/g vs (5.73±1.57)mg/g，P0.05]；左、右心室心肌组织AMPK含量无明显变化，但pAMPK含量显著降低，pAMPK/AMPK比值显著降低。结论 模拟高原5000m急性缺氧静息状态下心肌组织AMPK磷酸化水平降低，说明心肌能量供求处于平衡状态，心肌糖原含量增加可能是AMPK磷酸化水平降低的机制之一。 [关键词] 缺氧；心脏；糖原；腺苷酸活化蛋白激酶 [中图法分类号] [文献标志码] A Acute hypobaric hypoxia inhibites AMP-activated protein kinase phosphorylation in myocardium by increasing glycogen content in rats Shan Fabo, Cai Mingchun, Xu Gang, Yang Tao, Yin Jun, Huang Qingyuan (Department of Pathophysiology and High Altitude Physiology, Key Laboratory of High Altitude Medicine of Ministry of Education, Key Laboratory of High Altitude Physiology and High Altitude Disease of PLA, College of High Altitude Military Medicine, Third Military Medical University, Chongqing, 400038, China) [Abstract] Objective To determine the effects of acute hypobaric hypoxia on AMP-activated protein kinase (AMPK) phosphorylation and glycogen content in rat myocardium. Methods Totally 16 rats were randomly divided into control and acute hypoxia group (n=8). The rats in hypoxia group were submitted to simulated 5000 m of high altitude in a hypobaric chamber for 24 h. After the rats were sacrificed and their heart was taken out. Cardiac function and pulmonary artery pressure were measured, blood glucose and lactate as well as myocardial glycogen content was determined. Both ventricular myocardial GLUT4 protein and AMPK and phosphorylated AMPK (pAMPK) was detected by Western blotting.