菜豆普通细菌性疫病病原菌鉴定.pdfVIP

中国农业科学 2012,45(13):2618-2627 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.13.006 菜豆普通细菌性疫病病原菌鉴定 陈泓宇，徐新新，段灿星，王述民，朱振东 （中国农业科学院作物科学研究所/ 国家农作物基因资源与基因改良国家重大科学工程，北京 100081 ） 摘要：【目的】明确中国北方地区菜豆普通细菌性疫病病原菌。【方法】应用菌落特征、油菜黄单胞菌菜豆变 种诊断试剂检测、致病性测定、16S rDNA 和 16S-23S rDNA ITS 序列分析、特异 PCR 检测、脂肪酸分析及生理生 化反应特征对病原菌分离物进行鉴定。【结果】从病害样品和菜豆种子样品中均分离到类似黄单胞杆菌的细菌分离 物，选取 25 个代表分离物进行致病性测定，有 24 个分离物在菜豆品种“英国红”上导致典型菜豆普通细菌性疫 病症状。结合16S rDNA及16S-23S rDNA ITS 序列比对分析、特异性PCR 检测及生理生化反应的结果，24个分离 物中 7 株被鉴定为地毯草黄单胞菌菜豆变种，17 株为褐色黄单胞菌褐色亚种。【结论】中国北方地区发生的菜豆 普通细菌性疫病由地毯草黄单胞菌菜豆变种或（和）褐色黄单胞菌褐色亚种引起；褐色黄单胞菌褐色亚种在中国 属首次报道。 关键词：菜豆；菜豆普通细菌性疫病；地毯草黄单胞菌菜豆变种；褐色黄单胞菌褐色亚种 Identification of Pathogens Causing Common Bacterial Blight on Common Bean (Phaseolus vulgaris L.) CHEN Hong-yu, XU Xin-xin, DUAN Can-xing, WANG Shu-min, ZHU Zhen-dong (Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081) Abstract: 【Objective 】The objective of this study is to identify the pathogens that cause common bacterial blight on common bean in north China. 【Method 】 The causal agents were identified by colony characterization, detection using Xanthomonas campestris pv. phaseoli diagonsis kit (ADGEN EXPRESSTM), pathogenicity test, sequencing 16 rDNA and 16S-23S rDNA intergenic spacer (ITS) regions, PCR detection using specific primers for X. axonopodis pv. phaseoli and X .fuscans subsp. fuscans , fatty acid methyl esters (FAME) analysis and biochemical characterization tests. 【Result 】Bacterial isolates of Xanthmonads -like were isolated from all diseased plant and seed samples. Twenty-five