蜂王浆对雌性蜜蜂幼虫dynactin p62甲基化影响.pdfVIP

中国农业科学 2012,45(23):4909-4915 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.23.018 蜂王浆对雌性蜜蜂幼虫dynactin p62 甲基化影响 石元元，田柳青，张 飞，刘俊峰，颜伟玉，曾志将 （江西农业大学蜜蜂研究所, 南昌 330045 ） 摘要：【目的】研究蜂王浆对雌性蜜蜂幼虫dynactin p62 甲基化水平影响。【方法】以意大利蜜蜂（Apis mellifera ligustica ）和中华蜜蜂（A. cerana cerana）1 日龄幼虫为试验材料，分别饲喂意大利蜜蜂蜂王浆和 中华蜜蜂蜂王浆，并提取每组3 日龄和6 日龄幼虫样品的基因组DNA，利用Sequenom MassARRAY 技术检测dynactin p62 甲基化水平。【结果】饲喂异种蜂王浆可以更显著降低雌性蜜蜂幼虫dynactin p62 整体甲基化水平；饲喂同 一种蜂王浆，均为6 日龄幼虫dynactin p62 整体甲基化水平显著低于3 日龄；2 种蜂王浆对雌性蜜蜂幼虫dynactin p62 各位点甲基化水平影响不同。【结论】2 种蜂王浆对雌性蜜蜂幼虫dynactin p62 整体甲基化水平和各位点甲基 化水平影响不同，这说明不同蜂王浆具有不同的生物学效应。 关键词：蜜蜂；蜂王浆；dynactin p62；DNA 甲基化 Effect of Royal Jelly on the DNA Methylation of dynactin p62 in Female Honeybee Larvae SHI Yuan-yuan, TIAN Liu-qing, ZHANG Fei, LIU Jun-feng, YAN Wei-yu, ZENG Zhi-jiang (Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045) Abstract: 【Objective 】 The objective of this study is to analyze the influence of royal jelly on DNA methylation of dynactin p62 in female honeybee larvae. 【Method 】 One-day-old larvae of Apis mellifera ligustica and A. cerana cerana were used as materials, they were fed with royal jelly either from A. mellifera (RJM) or from A. cerana (RJC), and genome DNA was extracted from 3-and 6-day-old larvae, then DNA methylation of dynactin p62 was detected by Sequenom MassARRAY. 【Result 】The whole DNA methylation of dynactin p62 in female honeybee larvae decreased significantly after feeding with heterospecific royal jelly. The whole DNA methylation of dynactin p62 in 6-day-old larvae was significantly lower than 3-day-old larvae after feeding with homologous royal jelly. The methylated sit