甘蓝型油菜含油量及皮壳率的QTL分析.pdfVIP

中国农业科学 2007,40(4):677-684 Scientia Agricultura Sinica 甘蓝型油菜含油量及皮壳率的QTL分析 金梦阳，李加纳，付福友，张正圣，张学昆，刘列钊 （西南大学农学与生命科学学院，北碚 400715 ） 摘要：【目的】通过构建甘蓝型油菜遗传连锁图谱，对含油量及皮壳率进行QTL分析。【方法】以黄籽亲本GH 06 和黑籽亲本 P 杂交得到的 F 家系的 188 个株系为作图群体，利用 SRAP、SSR、AFLP 及 TRAP 四种标记构建遗传 174 2:6 连锁图谱，在此基础上采用复合区间作图法（CIM）对含油量及皮壳率两个性状进行 QTL 分析。【结果】图谱包含 19个连锁群、300个标记位点，总长为1 248.5 cM。共得到7 个与含油量相关的QTL，单位点遗传贡献率在3.73%～ 10.46%之间；4个与皮壳率相关的QTL，单位点遗传贡献率在4.89%～6.84%之间。【结论】黄籽油菜具有高含油量 的优势；皮壳率对含油量有显著影响；SRAP标记具有较好的检测QTL位点的能力。 关键词：甘蓝型油菜；遗传图谱；分子标记；数量性状位点；含油量；皮壳率 QTL Analysis of Oil and Hull Content in Brassica napus L. JIN Meng-yang, LI Jia-na, FU Fu-you, ZHANG Zheng-sheng, ZHANG Xue-kun, LIU Lie-zhao (College of Agronomy and Life Sciences, Southwest University, Beibei 400715) Abstract: 【Objective 】QTLs of oil and hull content were analyzed in Brassica napus L. by constructing a linkage map. 【Method 】F2:6 RIL population with 188 lines were derived from cross of GH06 ×P147 and used as mapping population. The SRAP, SSR, AFLP and TRAP markers were used to construct the linkage map. Composite interval mapping (CIM) was applied to identify quantitative trait loci associated with oil and hull content. 【Result 】300 markers were integrated into 19 linkage groups, covering 1248.5cM in total. Seven QTLs were found respinsible for oil content with single contribution to phenotypic variance ranging from 3.73% to 10.46%. Four QTLs were with hull content were found to have single contribution to phenotypic variance from 4.89% to 6.84%. 【Conclusion 】The yellow-seeded Brassica napus L. has in high oil content. The hull content has a great effect on the oil content.