甘蓝型油菜授粉功能缺失突变体及其 野生型柱头差异蛋白研究.pdfVIP

中国农业科学 2012,45(22):4728-4737 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.22.020 甘蓝型油菜授粉功能缺失突变体及其 野生型柱头差异蛋白研究 李春宏，付三雄，戚存扣 （江苏省农业科学院经济作物研究所/ 国家油菜改良中心南京分中心，南京210014 ） 摘要：【目的】基于蛋白质组学水平分析甘蓝型油菜野生型和授粉功能缺失突变体的柱头差异蛋白。【方法】 运用双向电泳技术研究甘蓝型油菜柱头授粉功能缺失的突变体FS-M 及其野生型（宁油10 号）柱头差异蛋白的表 1 达，结合质谱 （MALDI-TOF-TOF/MS）分析与蛋白质数据检索技术，鉴定甘蓝型油菜野生型柱头上调表达蛋白的性 质和功能。【结果】获得了甘蓝型油菜野生型柱头显著上调且可鉴定的蛋白有33 个，包括抗胁迫/防御、氧化还原 反应平衡调节、碳水化合物代谢、蛋白质水解、蛋白折叠、氨基酸和氮代谢、核苷酸代谢等7 类功能蛋白。【结论】 甘蓝型油菜野生型柱头中上调表达蛋白功能广泛，这些表达蛋白可能与甘蓝型油菜柱头授粉功能的调控有关。 关键词：甘蓝型油菜；柱头；双向电泳；质谱分析 Ananlysis of Differential Stigma Proteins Between a Pollination- Deficiency Mutant in Brassica napus L.and Its Wild Type LI Chun-hong, FU San-xiong, QI Cun-kou (Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Sub-center of National Rapeseed Development Center, Nanjing 210014) Abstract: 【Objective 】The difference in protein expression was identified between a pollination-deficiency mutant FS-M1 in Brassica napus L. and its wild type at proteomics level. 【Method 】 Two-dimensional electrophoresis (2-DE) was used to study the variation expression of stigma proteins between FS-M1 and its wild-type (NY10). The characteristics and functions of proteins expressed up-regulated in the B. napus L. stigma were analyzed with MALDI-TOF-TOF/MS and Protein Data Bank. 【Result 】 A total of 33 up-regulated proteins expressed in the wild-type stigma were identified, which were involved in 7 functional categories, including stress response and defense, regulation of redox homeostasis, carbohydrate met