芝麻空间诱变品系（种）的DNA指纹分析.pdfVIP

中国农业科学 2007,40(12):2696-2701 Scientia Agricultura Sinica 芝麻空间诱变品系（种）的DNA指纹分析 1 2 1 孙 建 ，涂玉琴 ，张秀荣 1 2 （中国农业科学院油料作物研究所，武汉 430062 ； 华中农业大学作物遗传改良国家重点实验室，武汉 430070 ） 摘要：【目的】揭示芝麻空间诱变品系（种）的 DNA 水平上的变异，探讨芝麻空间诱变的分子机理，以期为 作物空间诱变育种提供理论基础。【方法】首次应用 AFLP 标记对芝麻空间诱变的 3 个品系（种）及原始对照种豫 芝4号（未搭载处理的留地种子）进行DNA指纹分析。【结果】随机组合的30对AFLP引物对原始对照种及3个空 间诱变品系（种）进行扩增，共获得 1176 条 DNA 谱带，其中多态性谱带 625 条，多态性位点比率为 53.15%，每 对引物均扩增出多态性，并且每一个空间诱变品系（种）与原始对照种间均扩增出多态性 DNA 谱带。利用 NTSYS 软件进行类平均法（UPGMA）聚类发现3个空间诱变品系（种）明显地聚在一起，与原始对照种存在较大差异。【结 论】通过空间诱变能够在DNA水平上导致芝麻遗传物质变异，空间诱变是一种有效的育种途径。AFLP是一种研究 植物空间诱变材料的高效率的分子生物学方法。 关键词：芝麻；空间诱变；AFLP；DNA指纹 DNA Fingerprint Analysis in Space-induced Sesame Mutant Lines 1 2 1 SUN Jian , TU Yu-qin , ZHANG Xiu-rong 1 2 ( Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070) Abstract ：【Objective 】The objective of this report is to reveal the variations of space-induced sesame mutant lines in DNA level and discuss their molecular background in order to provide theoretical basis for crop space-flight mutation breeding. 【Method 】DNA fingerprint analysis was performed between three mutant lines and their original control variety Yuzhi No.4 by amplified fragments length polymorphism (AFLP) technology. 【Result 】Three mutant-lines and their parents were analyzed by randomly selected 30 pair of AFLP primers. Six hundred and twenty-five were polymorphic in 1176 DNA bands, w