植物多糖对乳猪肤缺损修复的实验研究.pdfVIP

摘 要 目的观察植物多糖对哺乳动物全层皮肤缺损修复的影响。 方法 选择乳猪 6 头（由成都中医药大学动物实验中心提供），体重 15-20KG/头，开展皮肤缺损实验：在乳猪肩胛后缘与髂骨前缘之间区域 背部切取直径为5.0cm的全层皮肤缺损创面，每侧各三个，分别使用植 物多糖（菟丝子总多糖和白芨多糖按比例混合）干粉、贝复新（重组牛 碱性成纤维细胞生长因子 bFGF ）、凡士林油纱处理创面。大体观察缺损 皮肤的修复生长,于 12 周末计算创面收缩率，切取损伤部位皮肤行组织 病理学检测，光镜下阅片。 结果 植物多糖组12 周时创面收缩率远较其余两对照组高，缺损修复区 皮肤组织病理学切片检测结果示：植物多糖组12 周时皮肤附属器修复、 纤维组织的增生、炎性细胞的浸润等与其余两组有显著性差异（P ＜ 0.01 ）；未在修复区皮肤发现神经元或神经纤维。 结论 植物多糖组在哺乳动物在体实验中可有效缩小创面，减少疤痕的 生成，促进毛囊和皮脂腺生长，创面毛细血管的增生，使胶原排列有序， 生长更成熟、粗大、接近正常的组织，抑制皮肤斑痕的发生，但菟丝子 总多糖未能在皮下诱导分化出神经元或促进神经纤维生长，尚不能完全 实现对全层皮肤缺损的功能性修复。 [关键词] 植物多糖 修复 乳猪 全层皮肤缺损 皮肤附属器 Abstract Objective: To observe the influence of the total polysaccharide from Chinese dodder seed (TPCD) and Polysaccharide from Chinese Bletillae rhizome(PCBR) on the healing of cutaneous deficiency. Methods: We cut off skin from scapular area of porkling with the anteroposterior diameter of 5.0cm.and use the powder of polysaccharide from Cuscuta and Bletillae rhizome, Vaseline gauze and bFGF (Recombinant Bovine Basic Fibroblast Grow) to treat the lesion part. Kill the porkling in the end of 12th month and take the lesion skin to do tissue pathology test. Results : Histopathology test shows that polysaccharide from Cuscuta and Bletillae rhizome group has obvious differences from the other two groups in the healing of hair follicles and the blood vessels in the 12 weeks’ treatment, and the recovery of cutaneous appendage and capillary vessels differs from the other two groups, but the fibroplasia, inflammatory cells’ infiltration show that all the three groups are same. Finally, polysaccharide from Cuscuta and Bletillae rhizome can’t induce the differentiation of subcutaneous nerve cell nor promote the nerve fiber’s growth. Conclusion