proteomics-chinese.ppt

 生命现象的物质基础是核酸和蛋白质，作为基因的载  体，核酸编制了所有的信息，而基因的表达产物蛋白 质，才是各种生物功能的执行者。 REAL COMPLEXITY… IS IN CELLULAR ROTEOMES BEYOND THE GENOME… Tissue Specific Expression Alternate Splicing, (1/3 of all genes) Post-Translational Modifications Types and Level: Signal Sequence cleavage Glycosylation Phosphorylation Farnylation Isoprenylation Acetylation All combine 100-1000 fold increase in complexity Future (challenging) research focus Proteome Functional Proteomics Solutions Different identification and separation techniques connected to meet different needs Alternative Selections Solutions Combination of instrumentation and protein chemistry Solutions Robust, Automation Proteomic techniques combined with bioinformatics for high-throughput data processing Solutions Fractionation, subcellular proteomics Reproducible control of sample preparation Proteome Research ESI-ion trap MS for Characterization of Proteins and peptide MS for Characterization of Proteins and peptide MS for Characterization of Proteins and peptide SEQUESTTM – How It Works Why Mass Spectrometry for Proteomics? Protein/Peptide Mass Accuracy Sequence Information Sensitivity Speed Direct Analysis of Protein Mixtures Post-translational Information Phosphorylation Glycosylation Quantitation (Expression) Questions 1. Wide Dynamic Range 2. Various Post-translation Modification Huge Amount of Species Four-dimensional Behaviour (3D Location, Timing) Cell Proteins Separation and Resolution Identification Structure and Function of proteins 蛋白质组学的主要研究方法 1、基于二维电泳-质谱技术的蛋白质组研究 2、基于质谱技术的蛋白质组研究 — 液相电泳-质谱技术 — 液相色谱-质联用技术 — Shotgun质谱技术 — ICAT技术 3、蛋白质芯片 High Vacuum System Inlet Ion Source Mass Filter Detector Data System Sample Target HPLC GC CE MALDI ESI Ion Spray API LSIMSS EI CI TOF