《lasma Ionization Source for Atmospheric Pressure Mass Spectrometry Imaging Using Near-Field Optical Laser Ablation》.ppt

Reporter:Hongbin Zou The Literature Report Date：2015-03-26 Maryia M. Nudnova, Je?ro?me Sigg, Pascal Wallimann, and Renato Zenobi* Plasma Ionization Source for Atmospheric Pressure Mass Spectrometry Imaging Using Near-Field Optical Laser Ablation Figure 1. Schematic of the SNOM-MS instrument. Ablation takes place in the optical near field on the sample at ambient conditions. The ablated neutral species are sampled with an active plasma sampling capillary and are transported into the mass spectrometer for chemical analysis. Figure 2. SEM images of etched tips fabricated by etching in one batch. The exit diameters of the tips is estimated to be (a) 400, (b) 450, and (c)～50 nm. Figure 3. Optical throughput measured for the three different SNOM tips shown in Figure 2. Figure 4. 50 × 50 μm area of the surface of a sinapic acid pellet scanned after 25 near-field laser ablation events with 85 nJ pulse laser energy each. (a) 3D view, (b) 2D view, and (c) depth profiles of the uppermost series of craters. The zero line in panel c represents the average height of the sinapic acid surface over the entire 50 × 50 μm scan, after flattening the image. Figure 5. (a) Amount of ablated substance of α-4-hydroxycyanocinnamic acid vs output laser energy for the three tips shown in Figure 3. (b) Amount of ablated substance vs fwhm of ablated craters. Tip #2 was used for all materials in Figure 5 (b). Representative error bars are indicated for the largest craters. These were calculated from the data, using the experimentally derived standard deviation, which was less than 20%, from 10 different measurements. Figure S1. SNOM image of a single crater ablated in 2,5 DHB pellet with following dimension: depth = 1.2 μm, FWHM = 6 μm, resulting in an estimated amount of ablated material of ~50 fmol. Figure S2. (a) Mass spectrum before ablation. (b) Mass spectrum of ~50 fmol of ablated material from the crater shown in Figure S1. m/z = 149 is a background signal, m/z=155 corresponds to protonate