白腐菌胞外液增效木质纤维素酶解产糖体系筛选与构建.pdfVIP

纤维素酶解产糖的要求，因此进一步探究了基质的不同预处理程度、不同酶负载对E taxodii 2538胞外粗蛋白复合酶解体系的影响。并探究了不同温度及不同粗蛋白添加比例对 解产糖效果的影响，对复合酶解体系进行了一定的优化。结果表明，在降低酶负载后，各 酶解体系葡萄糖产量均出现一定程度的下降，但上lacteus 254、E taxodii 2538胞外粗蛋白 复合体系葡萄糖产量的下降程度显著低于纯纤维素酶酶解体系，在酶负载降低50％后，三 者对中度碱处理及碱处理基质酶解葡萄糖产量分别降低了才11．34％、15．93％、23．82％与 7．75％、11．93％、14．82％，表明白腐菌的添加能够在一定程度上降低酶解体系对酶负载的 需求量。对复合酶解体系比例进行初步优化后，以E taxodii 2538胞外粗蛋白构建的复合 酶解体系最适比例为胞外粗蛋白总纤维素酶活力与商品纤维素酶活力的比值为l：3，最适 反应温度为48℃，在此条件下，48 h反应体系葡萄糖产量为624．52 mg／g基质，比对照提 高12．73％。 通过以上评估筛选及优化，得到了以白腐菌E taxodii 2538胞外粗蛋白与商品纤维素 酶构建的复合酶解体系，在降低纤维素酶用量的同时能够显著提高木质纤维素酶解葡萄糖 产量，具有一定的应用前景。 关键词：自腐菌，胞外液，纤维素酶，木质纤维素，酶解增效 Screening and Establishment of Lignocellulose Synergy Hydrolysis System with White-rot Fungi Extracellular Broth Master Candidate：Ni Hao--xiang Grade：20 1 2 Supervisor：Zhao Feng--li Major：Microbiology Research direction：Environmental Microbiology Abstract W11ite-rot fungi which have complete lignocellulose—degrading system have an advantage for the degradation of lignocellulose in natural state．Thus，it was possible to construct a complex enzyme hydrolysis system with the enzymes of white—rot fungi extracellular broth and commercial cellulases．ThiS work evaluated the effect of extracellular broth from 9 strains white—rot fungi and 1 strain brown·rot fungus on enzymatic hydrolysis of different kinds of lignocellulose，constructed the composite enzyme system with extracellular proteins from Echinodontium taxodii 2538 and cellulase．Further,the effects of temperature and ratio of composite enzyme system on enzymatic hydrolysis were also investigated,and then optimised the system． II This work first evaluated the activity of lignocellulose—degrade enzymes in the extracellular broth of 9 white-rot fungi strains and 1 brown—rot fungus strain，and evaluated the hydrolysis efficiency of extracellular broth of these strains．Then the effect of extracellular broth and extracellular proteins of these strains on enzymatic hydrolysis of different kinds of lingocellulose which came from different species was