《天然药物化学教学课件》Exp3.pptVIP

操作流程 取青菜叶 过滤 用20mL95%乙醇浸泡8h 静置分层 放出水层 加10mL石油醚 加50mLH2O 水 洗 醚 层 加无水硫酸钠干燥 湿法装柱 加样品 加石油醚-丙酮溶液洗脱 收集洗脱液 提取液置于分液漏斗 色谱分离图示 1. Purpose : Use column chromatography(CC) to separate the natural components from herbal materials. Learn to isolate phenols using CC. Be familiar with the examination of phenols. 2. Principle: EXP. 3 Application of silica gel column chromatography Protocatechuic aldehyde Protocatechuic acid ---The isolation of phenols from Ilex chinensis Sims 3. Procedures Filling column: fill little cotton in the bottom of column, add dry silica (6g, Grade V) into column. 2) Loadin