复方龙芩草微乳提取液有效成分体外抗H1N1流感病毒的药效研究.docVIP

复方龙芩草微乳提取液有效成分体外抗H1N1流感病毒的药效研究 　　摘要：目的 研究复方龙芩草微乳提取液中有效成分体外抗H1N1流感病毒增殖的作用，观察微乳提取液中有效成分对流感病毒的抑制率。方法 用均匀设计法安排实验，MTT法检测药物对H1N1流感病毒致A549细胞病变的抑制效率（ER），以ER为指标，运用Minitab17软件建立数学模型得出各因素之间的回归方程，分析各成分对ER的作用关系并优选出具有最佳抗H1N1流感病毒效果的有效成分比例。结果 复方成分中，黄芩苷发挥最显著的抗病毒作用，甘草苷对细胞病变有一定的抑制作用，5种成分对ER存在协同或制约性的关系。每毫升药液含甘草苷、黄芩苷、亮氨酸、天冬氨酸、谷氨酸分别为13.94 μg、49.44 μg、0.23 mg、1.25 mg、2.50 mg时ER值最佳，经验证ER值为85.34%±4.72%。结论 复方龙芩草微乳提取液有效成分的优化组合能较好发挥抗H1N1流感病毒的作用。 　　关键词：复方龙芩草；微乳提取液；均匀设计；H1N1流感病毒；A549细胞 　　DOI：10.3969/j.issn.1005-5304.2017.01.013 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）01-0049-06 　　Study on Anti-influenza Virus H1N1 Activity of Main Ingredients from Microemulsion Extract of Compound Longqincao in Vitro LIU Xiao-yan1， LI Yan1， LIU Shuai1， LI Wei-fei1， GU Li-gang2， LIU Xiao-ting2， DU Hong1 （1.School of Chinese Pharmacy， Beijing University of Chinese Medicine， Beijing 100102， China； 2. Educational Ministry Key Laboratory of Preventing and Treating Viral Diseases with Chinese Medicine， Beijing University of Chinese Medicine， Beijing 100029， China） 　　Abstract： Objective To study the effects of main ingredients from microemulsion extract of Compound Longqincao in vitro on anti-influenza virus H1N1 activity； To analyze effects of main ingredients from microemulsion extract on influenza virus inhibition rate. Methods Uniform design was used to conduct the experiment. MTT method was used to detect the effect rate （ER） of anti-influenza virus H1N1 on A549 cells. Setting ER as the index， Minitab17 software was used to establish mathematical model to come up with regression equations of all factors. The effects of ingredients on ER were analyzed and the efficient composition ratio of the optimum anti-influenza virus H1N1 was chosen. Results In the compound compatibility， baicalin showed the most obvious antivirus activity， and licorice glycosides had certain inhibition effects on pathological changes of cells. Five ingredients had coordinative or controlled relation with ER. When per milliliter l