基因芯片技术分析转SlNAC10基因拟南芥非生物胁迫相关差异表达基因.pdfVIP

中国细胞生物学学报 Chinese Journal of Cell Biology 2016, 38(8): 961–969 DOI: 10.11844/cj cb.2016.08.0016 基因芯片技术分析转SlNAC10基因拟南芥 非生物胁迫相关差异表达基因 焦 杨 于静洋 李倩倩 李秋莉* (辽宁师范大学生命科学学院, 辽宁省植物生物技术重点实验室, 大连116081) 摘要 NAC(NAM 、ATAF 1/2和CUC2)转录因子是植物特有的转录调控因子, 在植物的器官 建成、生长发育以及抵御非生物胁迫等方面发挥着至关重要的作用。该文利用基因芯片技术筛 选转SlNAC10基因拟南芥和野生型拟南芥非生物胁迫抗性相关差异表达基因, 并通过实时荧光定 量PCR对部分差异表达基因进行验证。芯片结果显示, 差异表达2倍以上的基因有4 054个, 其中与 非生物胁迫相关基因有15个, 与非生物胁迫相关的转录因子基因有14个, 这些基因参与应答渗透胁 迫、响应高盐、冷、热、高光强等胁迫。对差异表达2倍以上的基因进行GO(Gene Ontology)分析 和KEGG(Kyoto Encyclopedia of Genes and Genomes)分析, 发现这些基因在非生物胁迫相关的13个 注释中富集, 涉及相关代谢途径96个, 其中包括植物激素信号转导、精氨酸和脯氨酸代谢、吲哚生 物碱合成、谷胱甘肽代谢等。以上结果表明, SlNAC10可直接或间接调控多种下游基因的表达, 提 高植物抵御非生物胁迫的能力。 关键词 SlNAC10; 基因芯片; 非生物胁迫; 转基因拟南芥 Microarray Analysis of Differentially Expressed Genes Related to Abiotic Stress in SlNAC10-Transgenic Arabidopsis Jiao Yang, Yu Jingyang, Li Qianqian, Li Qiuli* (College of Life Science, Liaoning Normal University, Key Laboratory of Plant Biotechnology of Liaoning Province, Dalian 116081, China) Abstract NACs (NAM 、ATAF 1/2 and CUC2) were plant specific transcription factors and played essen- tial roles in plant morphogenesis, growth and development, and abiotic stress responses. In this study, microarray was used to analyze differentially expressed genes related abiotic stress in the wild-type (WT) and SlNAC10-transgenic Arabidopsis (L1), and the expression patterns of selected differentially expressed genes were further identified by quantitative Real-time PCR. A total of 4 054 differentially expressed genes were found, 15 of them were related to abiotic stress, and 14 were abiotic stress-related TFs. These genes were involved in responding to osmotic stress,