乳腺癌化学治疗敏感与耐药组织相关蛋白表达差异.pdfVIP

148 中南大学学报(医学版) J CentSou仇 Univ (Med Sci) DOI: 10.3969 Ij .issn.1672-7347.20 13.02.007 h忧p:1 I/xbwk/fìleup/PDF 1201302148.pdf 2013, 38(2) h忧p:; h位p: 乳腺癌化学治疗敏感与耐药组织相关蛋白表达差异 易文君，彭靖，张亚杰，付芬芬，邹球燕，唐圆圆 (中南大学湘雅二医院乳甲外科，长沙 41(011) [摘要]目的:研究乳腺癌化学治疗(化疗)敏感及耐药相关蛋白。方法:根据新辅助化疗效果筛选出化疗 敏感与耐药组，应用蛋白质组学技术研究两组间的差异蛋白并采用蛋白质印迹方法对部分差异蛋白进行验证。结 果:在化疗敏感组及耐药组乳腺癌组织蛋白质谱中找到 13 种差异蛋白质，其中 3 种在耐药组上调， 10 种下调。对 其中 7 种蛋白质进行验证，角蛋白 I 型细胞骨架 19(keratin type 1 cytoskeletal 19 ，阻C19)、胸昔磷酸化酶 (thymidine phosphorylase , TYPH) 在耐药组表达上调，而热休克蛋白 27(heat sl肌k protein 刀， HSP27)、角蛋白 I 型细胞骨 架 9(keratin 句pe 1 cytoskeletai 9，阻C9) 、胶原蛋白 α- 2( 六)链 [collagen alpha-2(VI) , C06A2]、波形蛋白 (vimentin ， VIME) 、 F 肌动蛋白 (actin cytoplasrnic 1, ACTB) 等表达下调，差异有统汁学意义 (PO.Ol)。结论:阻C19 和 TYPH 可 能与乳腺癌化疗耐药相关，而 HSP27，阻C9 ， C06A2, VIME 以及 ACTB 可能与化疗敏感相关。 [关键词]乳腺癌;蛋白质组;二维电泳;质谱;化学治疗;耐药 Differential protein expressions in breast cancer between drug sensitive tissues and drug resistant tissues YIWenjun, PENGJi吨， ZHANG Yajie, FU Fenfen, ZOU Q!ongyan, TANG Yuanyuan (Department of Breast and Thyroid Su伊 ABSTRACT Objective: To investigate the differential expression of the sensitive and resistant relative proteins in human hreast cancer tissue. Methods: A drug sensitive group and a 命ug resistant group for chemotherapy in patients with breast cancer were selected through neoadjuvant. The differential protein e叩ression in 2 groups was detected by proteomics techniques, and parts of differential proteins were identified by Western blot. Results:τhere were 13 differential proteins in the 2 groups, in which the expression of 3 proteins was up-regulated and 10 down-regulated. Seven proteins were identified by Western blot. The expression of keratin type 1 cytoskeletal 19 (阻Cl的， thymidine phosphorylase (TYPH) was up- regulated, and the expression ofheat shock protein 27 (HSP27), keratin type 1 cytoskeletal 9 (KIC9) , collagen 句ha刽VI) (C06剧， vimentin (VIME), and actin cytoplasmic 1 (ACTB) was 收稿曰期 (Date of reception): 2012-10-07 作者简介 (Bio伊.phy):