油松阔叶混交林不同层次优势植被根区土壤真菌的群落结构.pdfVIP

第 37 卷第 5 期 2ω9 年 5 月 东北林业大学学报 Vol. JI No.5 May 2ω9 JOVRNAL OF NORTHEA5T FOR立5TRY VNIVER5Iγy 油松阔叶混交林不同层次优势植被根区土壤真菌的群落结构1 ) 阮晓东 张惠文 孙冬雪‘ 蔡颖慧 李新宇 {中国科学院研究生院，北京， 1阳刚) (中国科学院沈阳应用生态研究所) (沈阳药科大学) (中国科学院研究生院) (中国科学院沈阳应用生态研究所} 摘 要采用具茵的 285 rDNA 的特异性~I物对千山沟松阔叶混交林中乔木层、灌木层和苹本层优势植被根 区土液中提取的...菌总 DNA 进行民;R 扩增，并通过 DGGE 技术对 PCR 产物进行分析，结果农明:乔木层针叶树 (油松、红松)根区土壤真筒的多样性最低，竿本层植栋栋区土壤共菌的多样性最高。乔木层阔叶树根区土壤，A.简 群落结构与灌木层相似，革本层与二者.?异较大。 关键词 千山:针阀混交林;梳被根区;真商;群落结构 ;PCR嗣DGGE 分类号引14.3 Fungal Community Structures in R，ωt Zone of Dominant Vegetations of Different Layers in Pinus ωbul?eformis Broad唰leaved Mixed Forest in Qiansban by PCR.DGG览/Ruan Xiωdong( Graduale Vniversity of Crunese Academy of Scienc咽. 8eijíng !C削49 ， P. 民. China); Zhang Huiwen( Instítute of Applíed Ecology , Chínese Aωdemy of 5ciences) ; Sun Dongxue( 5henyang Pharmaceutícal Vniversity) ; Cai Yinghui ( Graduate Vníversity of Chinese Academy of Sciences) ; Li Xinyu( Institute of Applied Ecology , Crunese Academy of Sciencω)/ /Joumal of North，ω5t Fol创世y Vniversity. - 2创)9， 37 (5). 48 -50 An experiment w部 conducted to study the structure of soíl fungal community in 1OOt zone of dominant vegetations of arhor , shruh and herb layers in Pinus tαbulαcformis broad.leaved mixed forest in Qianshan by PCR.DGGE. Partial genω from soil fungal community DNA were ampl凶ed using primers witrun 285 rDNA genes ín the fungi. The diversíty o(配比 amplified products wωdetected by denaturing gradient gel electrophoresis ( DGGE) . 民创ults showed that the soil fungal community of conífer in 盯bor layer h时 the lowest diversity , whíle the herb layer had the híghest diver百ity. The PCA analy- sis indicated that the structure of soil fungal community in 1OOt zone of broad.leaved trees in the arbor layer were similarω 也at in the shrub layer , and the herb layer had a great difference compared with the 111如f and shruh layers. Keywords Qianshan; Coníferous bro时.leaved mixed fo阳ts; Root zone; F山19i; Community struct阻四; PCR.肌GE 土壤中存在辛苦大量的微生物，这些微生物在植物凋落物 和根