美洲黑杨生长及基因表达差异.pdfVIP

第 38 眷第 8 期 2010 年 8 月 东北林业大学学报 JOURNAL OF NORTHEAST FORESTRY UNIVERSITY VQ1. 38 No.8 Aug. 2010 美洲黑杨生长及基因表达差异1 ) 黄秦军 袁定昌 赵自成 苏晓华 (中国林业科学研究院，北京， 1000(1) (北京市园林绿化局) (洞南省焦作?!?林科所) (中国林业科学研究院) 摘 票 以 9 年生关洲黑杨 50 号、36 号及 F1 子代为材料，对美洲黑杨亲子代的生长交井和杂种优势进行了 研究，并利用 mRNA 差异显示技术对美洲黑杨亲子代的基因在达差异进行研究。结果表明:羡洲黑杨 F1 子代间生 长贵存在丰富的遗伶变异，树高乎均变异系数是 24.59% ;胸径平均变异系数是 29.88% 。关洲1..杨 F1 子代在古5 期有 12.2i?岛的单株生长超过双亲，其乎均中亲优势和超亲优势分别为 42.05%和 32.51 i?岛;而 9 年生的中余优势和 起亲优势分别是 22.7% 和 12.0%0 美洲 J黑杨亲子代间的基因表达差异明显，共找到 57 个农达革并基因，农i在存 在着 3 种莹的差异类型和 6 种质的差异类型。在所有基因表达中父本特异表达2在鸥在杂种…代中是抑制型所占 比例最高 (2 1. 1%) ，子代特异农达总阁最少(5.3%) ，母本(50 号)基因对子代杂种优势的1贡献要大于义本。 关键词 关洲黑扬;mRNA 革，if. ji7F;!.因表达;杂种优势 分类号 5792 Differenωin Growtb and Gene E叩时ss in Populus delto协/日uang Qinjun( the Research Institute of Forestry , Chinese Academy of F ores町， Beijing 刷刷1 ， P. R. China); Yuan Dingchang( Beijing Gardening and Greening BUl四u); Zhao Zicheng( the F orestry Research Institute of Jiaozuo , Henan Province); Su Xiaohua ( the Research Institute of F orestry , Chinese Academy of Forestry)/ /Joumal of Northeast Forestry University. 呵2010 ， 38(8). 明 1 -3 ,10 The growth variation and heterosis in níne-year-old hybr训S of Popul!股份ltoides as well as the difIerence ín gene ex- pression were analy盼d by using Dífferential Display Reverse Transcription幡Polymerase Chain Reaction (DDRT♂CR) method. Re由ults showed that there were notíc巧able di他rences ín growth of the hybri巾， and the aver8tle coefficients of var- iation (CV) of tree h~ight and diameter at breast height were 24.59 percent and 29.88 percent r暗spectively. 币le height increment of 12.2 percent of individuals of F 1 hybrid progeny was significanùy higher than that of their parents , and the mi小parent heterosis (MPH) and over铺parent heterosis (OPM) 附陀 42.05 percent and 32. 51 percent , but those of nine- year翩。，}d F 1 hybrid pro伊Iy decreased 1022.7 percent and 12.0 阳rcent respectively. A significant difference in gene ex唰 press was observed between the hybrids and their parents ， ωd a