IL1基因多态性对胃粘膜TNFα、NFκB的表达的论文.docVIP

　　IL1基因多态性对胃粘膜TNFα、NFκB的表达的论文 【摘要】 目的探讨il1的基因多态性影响h.pylori感染后胃癌发生的机制。方法通过多态性分析和血清h.pylori抗体测定，收集105例癌低发区的学生作为研究对象，其中il1511t/t基因型32名（13名h.pylori+），c/c基因型36名（15名h.pylori+），c/t基因型37名（14名h.pylori+）。结果在胃体组织，h.pylori阴性个体tnfα和nfκb表达量极少，而且il1b511各基因型间也无显著差异。在h.pylori阳性个体，胃体部tnfα和nfκb mrna有较为明显的表达，il1b511t/t基因型个体比c/t和c/c基因型明显增加，分别为1.20±0.17 vs. 0.87±0.18和0.94±0.16；1.10±0.16 vs 0.90±0.15和0.97±0.17，f=33.4和12.9，p=0.0001和0.001。h.pylori阴性的nfκb mrna活性很低。在h.pylori阳性个体，nfκb 活性显著增加。而且il1b511t/t基因型个体比c/t和c/c基因型明显增加，分别为0.99±0.12vs 0.89±0.15和0.90±0.14，f=5.6，p=0.005。结论h.pylori感染促进tnfα和nfκb mrna表达，上调nfκb活性，在il1b511t/t基因型个体更明显。提示h.pylori感染后，存在il1b基因多态性→il1β↑→炎症细胞因子↑→胃癌发生的完整通路。. 【关键词】 胃癌；il1b；多态性；tnfα；nfκb；炎症 enhenced nfκb activity and tnfα transcript in h.pylori (+) individuals are associated orphisms cheng huanchao1, feng jueping2, ent of oncology,hubei cancer hospital，ail: ehusmn@yahoo..abstract：objective il1β gene polymorphisms affect the expression of of il1β. the induction of il1β by h.pylori strongly inhibited acid secretion,matory cytokine such as il1b and tnfa and activation of nfκb may be involved in il1b511 t allele associated gastric cancerogenesis.methodsone hundred and five healthy samples from the regions orphisms by pcrrflp. the expression of il1β, nfκb and tnfα easured using rtpcr, and nfκb activity sa.resultsin gastric corpus of h.pylori() individuals, the expression of nfκb and tnfα ilar and remarkably loong the h.pylori infected individuals, expressed higher level of nfκb and tnfα(1.20±0.17 vs. 0.87±0.18 and 0.94±0.16；1.10±0.16 vs. 0.90±0.15 and 0.97±0.17，f=33.4 and 12.9，p=0.0001 and 0.001,respectively) and induced the strongest nfκb activity (0.99±0.12 vs. 0.89±0.15 and 0.90±0.14，f=5.6，p=0.005) than that of other torphism and gastric cancerogenesis. key orphism; tnfα; nfκb;inflammatory 1资料与方法 1.1研究对象通过多态性分析和血清抗体测定，收集胃癌低发区（广东籍贯）的105例健康学生作为研究对象，无胃病史、无系统性红斑狼疮、糖尿病、类风湿性关节炎、炎症性肠病病史、无胃癌家族史。其中il1511t/t基因型32名（13名h.pylor