Determination of total flavonoids content of Prunella.docVIP

 PAGE \* MERGEFORMAT 13 Determination of total flavonoids content of Prunella Author: Deng Bin, Jiang Gang-Biao, Hong-Ying Huang, Chen-ping 6 [Abstract] Objective To study the visible and ultraviolet spectrophotometry of total flavonoids content of Prunella method. Visible spectrophotometric method is based on rutin as reference materials, aluminum nitrate chromogenic reagent, in the 510 nm wavelength determination of total flavonoids; UV spectrophotometric method is based on rutin as reference materials, at the 357nm wavelength determination of total flavonoids content. Results The average recovery was visible France 97.76%, 99.10% UV-law, the former RSD values are 1.02%, the latter of the RSD value is 0.88%. Conclusion UV spectrophotometry is simple, rapid, accurate and economical advantages, is an ideal in the determination of total flavonoids Prunella method. [Keywords:] Prunella vulgaris; total flavonoids; spectrophotometry Abstract: ObjectiveTo determine the total flavone content in Prunella vulgaris L. by VIS and UV Spectrophotometry.MethodsThe control sample of VIS Spectrophotometry was rutin, the revealing agent was Al (NO3) 3 and the analyzed wavelength was 510 nm.The control sample of UV Spectrophotometry was rutin and the analyzed wavelength was 357 nm.ResultsThe average recoveries of VIS and UV Spectrophotometry were 97.76% and 99.10%, the RSD were 1.02% and 0.88% respectively. ConclusionUV spectrophotometry is simple, rapid, accurate, economical and is suitable for content determination of total flavones in Prunella vulgaris L.. Keywords:: Prunella vulgaris L.; Total flavones; Spectrophotometry Traditional Chinese medicine for the Labiatae Prunella Prunella Prunella Prunella vulgaris L. Plant dry ear, are distributed in most parts of the country, easy cultivation, rich in resources. Contains mainly triterpenoids and their glycosides, phenylpropanoid classes, sterols and their glycosides, flavonoids, coumarins, organic acids, volatile oil and