Fluorimetric Determination of the optimal activity of aldose reductase.docVIP

 PAGE \* MERGEFORMAT 14 Fluorimetric Determination of the optimal activity of aldose reductase Study: Xiao Wang was Fanghui Long Peace Valley section of hairs Juan Li Ling Ouyang Dong-sheng [Abstract] Objective To find the optimal generating fluorescence spectrometry NADP erythrocyte aldose reductase (AR) activity in the program. Methods of hemoglobin concentration, fluorescence excitation NADP solution storage bath time and time on fluorescence intensity, determination of optimal activity of erythrocyte AR The NADP generation method, this method of determination of AR activity in normal and diabetic rats. Results NADP generated by the hemoglobin method of accuracy the impact of NADP in the termination of the solution after the reaction by adding 250 l 6% perchloric acid precipitation to eliminate the effects of hemoglobin , water bath induced fluorescence time 5,30,60 min, fluorescence was no difference in the coefficient of variation in the reaction system before excitation fluorescence generated NADP solution stored at -20 condition stable for at least 4 w. AR activity in diabetic rats significantly higher than the control group (P lt;0.05). Conclusion NADP optimization method can produce a more accurate determination of erythrocyte AR activity. [Keywords:] diabetes, aldose reductase, fluorescence, activity [Abstract] Objective To optimize the the NADP generating fluorimetric method for aldose reductase (AR) activity. Methods The effect of haemoglobin, the time of aqueous bath arousing fluorescence and storage time of NADP solution on the fluorescent value was observed. It was optimized that the NADP generating fluorimetry assayed erythrocytes AR activity. AR activity of normal and diabetic mellitus rat were examined.Results The accuracy of this assay was influenced by hemoglobin concentration which could be eliminated by adding 250 l 6% perchloric acid to precipitate hemoglobin as the reaction was terminated. It had no difference for the variat