HPLC determination of metformin in human plasma levels of.docVIP

 PAGE \* MERGEFORMAT 4 HPLC determination of metformin in human plasma levels of [Abstract] Objective To determine the concentration of metformin in human plasma by HPLC-UV method. Methods perchlorate direct precipitation, measured plasma concentrations of metformin. Of impurities do not interfere with the results of plasma samples of peak 1.25,0.125 and 0.025μ g/ml the recovery rates were 93.9%, 92.6% and 91.4%; 1.250,0.25 and 0.025μ g/ml intraday variations are as follows: 1.33%, 4.08% and 7.94%; day variation were: 4.56%, 2.78% and 2.19%; the linear range of 0.0125 ~ 2.50μ g/ml, the minimum detection limit 0.0125μ g/ml; plasma metformin stability of freeze-thaw test. Conclusion The method is stable and good precision, accurate and reliable for determination of plasma concentrations of metformin. [Keywords:] metformin; high-performance liquid chromatography Metformin (metformin) is a biguanide oral hypoglycemic agents, its primary role in the islets outside the organization, inhibit intestinal absorption of glucose and increase peripheral tissue glucose utilization, reduction of liver glycogen xenobiotic to achieve the purpose of lowering blood glucose for type 2 diabetes mellitus patients. Reported in the literature commonly used detection method is high-performance liquid chromatography [1 ~ 3], commonly used as the internal standard atenolol [1,3]. Often join the mobile phase ion-pair reagent, such as sodium dodecyl sulfate [1] heptane-sodium [2] and octane sulfonate [4]. In this paper, a kind of in the mobile phase by adding sodium lauryl sulfate, using perchloric acid precipitation directly measured the concentration of a simple method. An experimental material 1.1 Test drugs and reagents metformin Standard: China’s pharmaceutical and biological products available. Acetonitrile (HPLC pure): Fisher Company, Inc, USA; the other reagents were commercially available analytical pure. 1.2 Instrument Shimadzu high-performance liquid chromatograp