HPLC Determination of particle VITA Participation levels of vitamin B2.docVIP

 PAGE \* MERGEFORMAT 7 HPLC Determination of particle VITA Participation levels of vitamin B2 Author: Bowen Kui, Du, Duan Guang-Zhen, Zhang Xiaoyu, Zhang M [Keywords:] HPLC; Weitashen Granules; Vitamin B2 VITA Participation particles from ginseng, hawthorn, and the composition of a variety of vitamins, vitamin B2, one of the side of the active ingredient, vitamin B2 Many important coenzyme in the body components. Vitamin B2 content determination methods are fluorescence spectrophotometry, etc. I used HPLC determination of its content through the study methodology, its precision, repeatability, stability, plus recoveries are in line with requirements for participation particle VITA provides the experimental basis for quality control in order to effectively control its quality. An instrument and reagent reference substance of vitamin B2 (100369-200301 for the determination of use) purchased in China’s pharmaceutical and biological products, purity greater than 98.5%. Victoria, he made reference particles, Agilent 1100 series high-performance liquid chromatography (G1311A Quat Pump, G1314A VWD, G1379A DEGASSER); Agilent 1100 workstation; CP225D (sartorius) one hundred thousandth scale; AS3120A ultrasonic cleaner; U-3010 UV-VIS double-beam scanning spectrophotometer. Chromatography pure acetonitrile, water re-distilled water; other reagents were analytical pure. Two experimental conditions 2.1 The selection of chromatographic conditions Column: Agilent XDB-C18 (5 μ m, 150 mm * 4.6 mm) Mobile phase: acetonitrile and -0.008 mol / L hexane sulfonate - triethylamine - acetic acid (12:87:0.25: 0.75). Detection wavelength: 246 nm; column temperature: 30 ℃ ; flow rate: 1 ml / min; theoretical plate number calculated by vitamin B2 reference substance of not less than 5000 peaks. 2.2 Determination of wavelength selection to take a small amount of vitamin B2, standard solution, dilution, home U-3010 UV-VIS dual-beam scanning spectrophotometer, under the 200 ~ 400 nm