HPLC determination of puerarin in lipid-lowering diet particle content of.docVIP

 PAGE \* MERGEFORMAT 8 HPLC determination of puerarin in lipid-lowering diet particle content of Authors: LAM Siu, Yu-Fang, Nanmin Lun, WANG Tie-zhu, ZHAO Quan Cheng [Keywords:] high performance liquid chromatography Abstract: Objective To establish the lipid-lowering diet in particle method for the determination of puerarin. Methods Shim-pack (Shimadzu) ODS C column (150 mm * 4.6 mm, 5 μ m), mobile phase was methanol 0.1% phosphoric acid (25.3:74.7), flow rate of 0.9 mg / ml, detection wavelength was 250 nm. The results of puerarin in the range of 0.114 0 0.570 0 a good linear relationship, r = 0.999 6, the average recovery was 98.7%, RSD of 0.67%. Conclusion The measurement method is accurate, reproducible, can be used as quality control agents. Keywords:: lipid-lowering diet particle; puerarin; high performance liquid chromatography Determination of Puerarin in Jiangzhijianfei Granule by HPLC Abstract: ObjectiveTo establish the HPLC method for the determination of puerarin in Jiangzhijianfei granule.MethodsThe chromatographic conditions included: The column is Shim-pack ODS C18 column (150 mm * 4.6 mm, 5 μ m), the mobile phase is methol-water H3PO4, the detection wavelength is 250 nm and the column temperature is room temperature.ResultsThe linear range of puerarin was 0.114 0 ~ 0.570 0 μ g, r = 0.999 6.The average recovery of puerarin was 98.7% with RSD 0.67%. ConclusionThe mothod is accurate and can be used as a determination of puerarin in Jiangzhijianfei granule. Keywords:: Jiangzhijianfei granule; Puerarin; HPLC Lipid-lowering diet particles are in the original ministerial standard 17 received lipid-lowering diet containing the drug developed on the basis chip made of traditional Chinese medicine, natural medicine, nine categories of new drugs, from the fleece-flower root, 37, puerarin, dodder seed, wolfberry fruit, pine pollen, Salvia , rhubarb, Alisma, such as traditional Chinese medicine composed of Artemisia. The drug has a nourishing liver