HPLC determination of qi fluid capsule contents Schisandrin.docVIP

 PAGE \* MERGEFORMAT 5 HPLC determination of qi fluid capsule contents Schisandrin [Keywords:] Schisandra, Qi Sheng Jin capsule, high performance liquid chromatography Qi fluid capsule by the ginseng, Ophiopogon, Schisandra, Astragalus, Polygonatum, liquorice, jujube composed of 7 herbs. Schisandra is one of the main drugs, Schisandra schisandrin is the main active ingredient. In this paper, high performance liquid chromatography in the preparation to determine the content of schisandrin, provide the basis for its quality control. An instrument and reagent Agilent 1100 HPLC system the United States and its workstation. Methanol HPLC grade water for ultra-pure water, schisandrin standard (provided in the examination, with determination, batch number 110536200306, Qi Sheng Jin capsules (made lot number 080810,080814,080817, specifications for the 0.45g / tablets. 2 Methods and Results 2.1 Chromatographic conditions Agilent 1100 HPLC system, column: Symmetry C18 (4.6 250mm, 5m, detector: VWD, mobile phase: methanol water (60:40, flow rate 1.0ml/min, column temperature: 25 , the detection wavelength : 250nm, injection volume: 10l. 2.2 Preparation of the solution The test solution: fine to take capsule contents 50.01g, home 100ml volumetric flask, add methanol 60ml ultrasonic 30min, let cool, add methanol to the mark, shake and dumped the beginning of the filtrate, the filtrate collected, filtered with a 0.45m pore membrane filtration, the filtrate as the test solution. negative control solution: fine to take other medicines without Schisandra 50.02g, according to the method of operation, made negative reference solution. reference solution: accurately weighed less at 60 4h pressure drying the reference standard schisandrin 14.5mg, home 50ml volumetric flask, add methanol and set the scale, shake, as the reference solution. 2.3 System suitability test Control solution were taken for the test solution, 10l of the negative control so