HPLC determination of Qingkailing geniposide capsule content.docVIP

 PAGE \* MERGEFORMAT 8 HPLC determination of Qingkailing geniposide capsule content Of: Huang Xiaodan, Fu Ying, Chen Hei Ling, Luo Jianming [Abstract] Objective To develop a HPLC method for the determination Qingkailing geniposide content of capsules. Methods Agilent Agilent 1100 high performance liquid chromatography, Gracesmart C18 column and detection wavelength 238 nm, acetonitrile - water (15: 85) as the mobile phase, flow rate 1.0 mL / min. the results of the linear range of 0.060 4 ~ 0.302 0 g (r = 0.999 8), the average recovery was 98.73% (n = 10), RSD = 1.61%. Conclusion The method accurate and reliable, can be used for capsule Qingkailing geniposide content. [Key Words] high performance liquid chromatography, Qingkailing capsule, geniposide, determination Abstract: Objective To establish a HPLC method for determination of geniposide content in Qingkailing Capsule. Methods HPLC with Gracesmart C18 was used and detection wavelength was 238 nm with acetonitrile-water (15:85) as mobile phase. The flow rate was 1.0 mL / min. Results The standard curve was linear over the range of 0.060 4 ~ 0.302 0 μg (r = 0.999 8). The average recovery was 98.73%, and RSD was 1.61%. Conclusion This method is reliable, accurate and suitable for the determination of geniposide in Qingkailing Capsule. Keywords:: HPLC, Qingkailing Capsule, geniposide, content determination Qingkailing capsule by the honeysuckle, Radix, gardenia, buffalo horn, acid etc., clearing and detoxifying effect, treating upper respiratory tract infection, viral infection, high fever embolism. Geniposide Gardenia is the main active one of the components, the quality of Qingkailing capsule collection is not currently set the standard determination method geniposide, in order to better control drug quality, we used high performance liquid chromatography (HPLC) method for the determination of the preparation in the content Geniposide experiment The results show that the separation of high