HSPN serum TNF TGF1 and IL6 expression and clinical significance.docVIP

 PAGE \* MERGEFORMAT 8 HSPN serum TNF TGF1 and IL6 expression and clinical significance [Keywords:] purpura nephritis; of tumor necrosis factor (TNF) @; TGF 1; interleukin (IL) 6; ELISA Henoch-Schonlein purpura is a common allergic capillary bleeding disorders, the clinical features of non-thrombocytopenic purpura, purpura nephritis and its serious complications, the prognosis is poor. Studies have shown that inflammatory cytokines in purpura Abnormal expression of nephritis patients. Tumor necrosis factor @ (TNF @) and interleukin 6 (IL 6) is pro-inflammatory cytokines, in allergic purpura play an important role (1); and transforming growth factor 1 ( TGF 1) is a hormone-like activity of the peptide, exists in the megakaryocyte and platelet a granules, the process in the development of purpura has a role in promoting (2). This paper purpura nephritis observed in serum of patients TNF @, TGF 1 and the expression of IL 6 and its clinical significance. 1 Materials and methods 1.1 Clinical material from January 2008 to December 2009 in our hospital diagnosed 53 cases of purpura nephritis, of which 29 males and 25 females, aged 24 to 68 (average 42.1) years, pre-test were not receiving hormone and immune inhibitor treatment. The other 40 cases selected serum HSP patients and 40 normal adult serum as a control group .3 group sex, age, weight and other factors had no significant difference between comparable. 1.2 TNF @, TGF 1 and IL 6 test group and purpura anaphylactoid purpura nephritis patients in the diagnosis of fasting venous blood the next morning 10 ml, after by the sodium citrate to 3 000 r / min centrifugation 10 min, specimens from plasma, was frozen at minus 20 refrigerator reserve; normal control group, blood sampling and preservation methods above, and are in the blood concentration after the test within one month. application of ELISA (ELISA) Serum in TNF @, TGF 1 and IL 6 expression. concrete steps: coating antigen: diluted with coati