Hypoxia promotes cultured astrocytes in the mechanism of -catenin accumulation.docVIP

 PAGE \* MERGEFORMAT 16 Hypoxia promotes cultured astrocytes in the mechanism of -catenin accumulation Of: Cui Xiaoping, Lin Hang, The Relationship Between Military Mu Shan, Ye Jianxin, Yan Hongbing, should the King, [Abstract] Objective To investigate hypoxia on astrocytes in the mechanism of -catenin accumulation. Methods mice were isolated hippocampal astrocytes, were placed under normoxic and hypoxic conditions, 12h and 24h after training, Using RT-PCR and Western blot, Wnt pathway in astrocytes associated protein Wnt3, Wnt3a and phosphatidylinositol -3 kinase / Akt (PI3K/Akt) pathway, phosphorylated Akt (p-Akt) and phosphorylated glycogen synthase kinase-3 (p-GSK-3) protein expression. Results normoxic astrocytes cultured hippocampal memory in the Wnt3 expression of hypoxia on hippocampal astrocytes Wnt3 expression was not effect (Pgt; 0.05); normoxia and hypoxia in hippocampal astrocytes do not exist in Wnt3a expression; hypoxic astrocytes in the hippocampus increased p-Akt and p-GSK-3 protein levels (P lt;0.05). hypoxic hippocampal astrocytes by increasing the p-Akt protein and p-GSK-3 protein levels for -catenin accumulation. [Keywords:] hypoxia; astrocytes; Wnt; -catenin; Akt; GSK-3 ABSTRACT: ObjectiveTo explore the mechanism of hypoxia-promoted accumulation of -catenin in astrocytes.MethodsAstrocytes were isolated, cultured and identified from Kunming mouse hippocampus in vitro, then cultured in hypoxia and traditional oxygen for 24 hours and 12 hours, respectively. The expressions of Wnt3 and Wnt3a in hippocampus astrocytes were detected by RT-PCR. The effect of hypoxia on the expressions of Wnt3 and Wnt3a in hippocampus astrocytes was analyzed by RT-PCR. The influence of hypoxia on the expressions of phosphorylated Akt and glycogen synthase kinase- 3 (GKS-3) protein was analyzed by Western blot.ResultsWnt3 was expressed in hippocampus astrocytes and hypoxia did not affect its expression (Pgt; 0.05). Wnt3a was not detected in hippocampus